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Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost

Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected...

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Autores principales: Ravi, Krithika, García-Hidalgo, Javier, Gorwa-Grauslund, Marie F, Lidén, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486835/
https://www.ncbi.nlm.nih.gov/pubmed/28299400
http://dx.doi.org/10.1007/s00253-017-8211-y
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author Ravi, Krithika
García-Hidalgo, Javier
Gorwa-Grauslund, Marie F
Lidén, Gunnar
author_facet Ravi, Krithika
García-Hidalgo, Javier
Gorwa-Grauslund, Marie F
Lidén, Gunnar
author_sort Ravi, Krithika
collection PubMed
description Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a culture collection. The specific growth rates on benzoate, p-coumarate, and 4-hydroxybenzoate were considerably higher (0.26–0.27 h(−1)) than those on ferulate and vanillate (0.21 and 0.22 h(−1)), as were the uptake rates. There was no direct growth of P. putida KT2440 on vanillin, but instead, vanillin was rapidly converted into vanillate at a rate of 4.87 mmol (g(CDW) h)(−1) after which the accumulated vanillate was taken up. The growth curve reflected a diauxic growth when mixtures of the model compounds were used as carbon source. Vanillin, 4-hydroxybenzoate, and benzoate were preferentially consumed first, whereas ferulate was always the last substrate to be taken in. These results contribute to a better understanding of the aromatic metabolism of P. putida in terms of growth and uptake rates, which will be helpful for the utilization of these bacteria as cell factories for upgrading lignin-derived mixtures of aromatic molecules. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00253-017-8211-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-54868352017-07-11 Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost Ravi, Krithika García-Hidalgo, Javier Gorwa-Grauslund, Marie F Lidén, Gunnar Appl Microbiol Biotechnol Applied Microbial and Cell Physiology Starting from mature vegetable compost, four bacterial strains were selected using a lignin-rich medium. 16S ribosomal RNA identification of the isolates showed high score similarity with Pseudomonas spp. for three out of four isolates. Further characterization of growth on mixtures of six selected lignin model compounds (vanillin, vanillate, 4-hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a culture collection. The specific growth rates on benzoate, p-coumarate, and 4-hydroxybenzoate were considerably higher (0.26–0.27 h(−1)) than those on ferulate and vanillate (0.21 and 0.22 h(−1)), as were the uptake rates. There was no direct growth of P. putida KT2440 on vanillin, but instead, vanillin was rapidly converted into vanillate at a rate of 4.87 mmol (g(CDW) h)(−1) after which the accumulated vanillate was taken up. The growth curve reflected a diauxic growth when mixtures of the model compounds were used as carbon source. Vanillin, 4-hydroxybenzoate, and benzoate were preferentially consumed first, whereas ferulate was always the last substrate to be taken in. These results contribute to a better understanding of the aromatic metabolism of P. putida in terms of growth and uptake rates, which will be helpful for the utilization of these bacteria as cell factories for upgrading lignin-derived mixtures of aromatic molecules. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00253-017-8211-y) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-03-15 2017 /pmc/articles/PMC5486835/ /pubmed/28299400 http://dx.doi.org/10.1007/s00253-017-8211-y Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Applied Microbial and Cell Physiology
Ravi, Krithika
García-Hidalgo, Javier
Gorwa-Grauslund, Marie F
Lidén, Gunnar
Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
title Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
title_full Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
title_fullStr Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
title_full_unstemmed Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
title_short Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost
title_sort conversion of lignin model compounds by pseudomonas putida kt2440 and isolates from compost
topic Applied Microbial and Cell Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486835/
https://www.ncbi.nlm.nih.gov/pubmed/28299400
http://dx.doi.org/10.1007/s00253-017-8211-y
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