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Potential Activity of Subglacial Microbiota Transported to Anoxic River Delta Sediments

The Watson River drains a portion of the SW Greenland ice sheet, transporting microbial communities from subglacial environments to a delta at the head of Søndre Strømfjord. This study investigates the potential activity and community shifts of glacial microbiota deposited and buried under layers of...

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Autores principales: Cameron, Karen A., Stibal, Marek, Olsen, Nikoline S., Mikkelsen, Andreas B., Elberling, Bo, Jacobsen, Carsten S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486838/
https://www.ncbi.nlm.nih.gov/pubmed/28070677
http://dx.doi.org/10.1007/s00248-016-0926-2
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author Cameron, Karen A.
Stibal, Marek
Olsen, Nikoline S.
Mikkelsen, Andreas B.
Elberling, Bo
Jacobsen, Carsten S.
author_facet Cameron, Karen A.
Stibal, Marek
Olsen, Nikoline S.
Mikkelsen, Andreas B.
Elberling, Bo
Jacobsen, Carsten S.
author_sort Cameron, Karen A.
collection PubMed
description The Watson River drains a portion of the SW Greenland ice sheet, transporting microbial communities from subglacial environments to a delta at the head of Søndre Strømfjord. This study investigates the potential activity and community shifts of glacial microbiota deposited and buried under layers of sediments within the river delta. A long-term (12-month) incubation experiment was established using Watson River delta sediment under anaerobic conditions, with and without CO(2)/H(2) enrichment. Within CO(2)/H(2)-amended incubations, sulphate depletion and a shift in the microbial community to a 52% predominance of Desulfosporosinus meridiei by day 371 provides evidence for sulphate reduction. We found evidence of methanogenesis in CO(2)/H(2)-amended incubations within the first 5 months, with production rates of ~4 pmol g(−1) d(−1), which was likely performed by methanogenic Methanomicrobiales- and Methanosarcinales-related organisms. Later, a reduction in methane was observed to be paired with the depletion of sulphate, and we hypothesise that sulphate reduction out competed hydrogenotrophic methanogenesis. The structure and diversity of the original CO(2)/H(2)-amended incubation communities changed dramatically with a major shift in predominant community members and a decline in diversity and cell abundance. These results highlight the need for further investigations into the fate of subglacial microbiota within downstream environments. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00248-016-0926-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-54868382017-07-11 Potential Activity of Subglacial Microbiota Transported to Anoxic River Delta Sediments Cameron, Karen A. Stibal, Marek Olsen, Nikoline S. Mikkelsen, Andreas B. Elberling, Bo Jacobsen, Carsten S. Microb Ecol Note The Watson River drains a portion of the SW Greenland ice sheet, transporting microbial communities from subglacial environments to a delta at the head of Søndre Strømfjord. This study investigates the potential activity and community shifts of glacial microbiota deposited and buried under layers of sediments within the river delta. A long-term (12-month) incubation experiment was established using Watson River delta sediment under anaerobic conditions, with and without CO(2)/H(2) enrichment. Within CO(2)/H(2)-amended incubations, sulphate depletion and a shift in the microbial community to a 52% predominance of Desulfosporosinus meridiei by day 371 provides evidence for sulphate reduction. We found evidence of methanogenesis in CO(2)/H(2)-amended incubations within the first 5 months, with production rates of ~4 pmol g(−1) d(−1), which was likely performed by methanogenic Methanomicrobiales- and Methanosarcinales-related organisms. Later, a reduction in methane was observed to be paired with the depletion of sulphate, and we hypothesise that sulphate reduction out competed hydrogenotrophic methanogenesis. The structure and diversity of the original CO(2)/H(2)-amended incubation communities changed dramatically with a major shift in predominant community members and a decline in diversity and cell abundance. These results highlight the need for further investigations into the fate of subglacial microbiota within downstream environments. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00248-016-0926-2) contains supplementary material, which is available to authorized users. Springer US 2017-01-09 2017 /pmc/articles/PMC5486838/ /pubmed/28070677 http://dx.doi.org/10.1007/s00248-016-0926-2 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Note
Cameron, Karen A.
Stibal, Marek
Olsen, Nikoline S.
Mikkelsen, Andreas B.
Elberling, Bo
Jacobsen, Carsten S.
Potential Activity of Subglacial Microbiota Transported to Anoxic River Delta Sediments
title Potential Activity of Subglacial Microbiota Transported to Anoxic River Delta Sediments
title_full Potential Activity of Subglacial Microbiota Transported to Anoxic River Delta Sediments
title_fullStr Potential Activity of Subglacial Microbiota Transported to Anoxic River Delta Sediments
title_full_unstemmed Potential Activity of Subglacial Microbiota Transported to Anoxic River Delta Sediments
title_short Potential Activity of Subglacial Microbiota Transported to Anoxic River Delta Sediments
title_sort potential activity of subglacial microbiota transported to anoxic river delta sediments
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486838/
https://www.ncbi.nlm.nih.gov/pubmed/28070677
http://dx.doi.org/10.1007/s00248-016-0926-2
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