Cargando…

Novel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization

A novel method for indirect determination of MCPD esters levels in lipid samples has been developed. The method is based on combination of extraction and derivatization in the same sample preparation step. It is achieved by the application of diethyl ether as extraction solvent for isolation of anal...

Descripción completa

Detalles Bibliográficos
Autores principales: Jędrkiewicz, Renata, Głowacz-Różyńska, Agnieszka, Gromadzka, Justyna, Konieczka, Piotr, Namieśnik, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486935/
https://www.ncbi.nlm.nih.gov/pubmed/28484809
http://dx.doi.org/10.1007/s00216-017-0381-z
_version_ 1783246363500216320
author Jędrkiewicz, Renata
Głowacz-Różyńska, Agnieszka
Gromadzka, Justyna
Konieczka, Piotr
Namieśnik, Jacek
author_facet Jędrkiewicz, Renata
Głowacz-Różyńska, Agnieszka
Gromadzka, Justyna
Konieczka, Piotr
Namieśnik, Jacek
author_sort Jędrkiewicz, Renata
collection PubMed
description A novel method for indirect determination of MCPD esters levels in lipid samples has been developed. The method is based on combination of extraction and derivatization in the same sample preparation step. It is achieved by the application of diethyl ether as extraction solvent for isolation of analytes released from esterified forms from the water phase and as dilution solvent for solid PBA – the derivatization agent. It is a noteworthy improvement of recommended indirect approaches available in the literature because such steps as sample clean-up, multiple liquid–liquid extractions, and preconcentration are excluded in the proposed solution. In this way, the developed procedure is shortened and simplified. Such an approach also minimizes the utilization of organic solvents; therefore, it is in accordance with the principles of “green analytical chemistry.” In spite of the fact that the step of sample clean-up is omitted, no deterioration in GC-MS system performance was observed. Equivalence testing of the developed procedure and AOCS cd 29b-13 official method (SGS) has been conducted. It was concluded that results obtained by both methods do not significantly differ statistically. The procedure has been applied to determination of MCPD esters concentrations in lipid fractions isolated by accelerated solvent extraction technique from such foodstuffs as bakery products, salty deep-fried snacks, and instant products. In all investigated samples, the level of bound MCPD was elevated. Additionally, for both procedures, the environmental impact (with the use of analytical Eco-scale) and uncertainty budget have been assessed and compared.
format Online
Article
Text
id pubmed-5486935
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-54869352017-07-17 Novel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization Jędrkiewicz, Renata Głowacz-Różyńska, Agnieszka Gromadzka, Justyna Konieczka, Piotr Namieśnik, Jacek Anal Bioanal Chem Research Paper A novel method for indirect determination of MCPD esters levels in lipid samples has been developed. The method is based on combination of extraction and derivatization in the same sample preparation step. It is achieved by the application of diethyl ether as extraction solvent for isolation of analytes released from esterified forms from the water phase and as dilution solvent for solid PBA – the derivatization agent. It is a noteworthy improvement of recommended indirect approaches available in the literature because such steps as sample clean-up, multiple liquid–liquid extractions, and preconcentration are excluded in the proposed solution. In this way, the developed procedure is shortened and simplified. Such an approach also minimizes the utilization of organic solvents; therefore, it is in accordance with the principles of “green analytical chemistry.” In spite of the fact that the step of sample clean-up is omitted, no deterioration in GC-MS system performance was observed. Equivalence testing of the developed procedure and AOCS cd 29b-13 official method (SGS) has been conducted. It was concluded that results obtained by both methods do not significantly differ statistically. The procedure has been applied to determination of MCPD esters concentrations in lipid fractions isolated by accelerated solvent extraction technique from such foodstuffs as bakery products, salty deep-fried snacks, and instant products. In all investigated samples, the level of bound MCPD was elevated. Additionally, for both procedures, the environmental impact (with the use of analytical Eco-scale) and uncertainty budget have been assessed and compared. Springer Berlin Heidelberg 2017-05-08 2017 /pmc/articles/PMC5486935/ /pubmed/28484809 http://dx.doi.org/10.1007/s00216-017-0381-z Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Paper
Jędrkiewicz, Renata
Głowacz-Różyńska, Agnieszka
Gromadzka, Justyna
Konieczka, Piotr
Namieśnik, Jacek
Novel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization
title Novel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization
title_full Novel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization
title_fullStr Novel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization
title_full_unstemmed Novel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization
title_short Novel fast analytical method for indirect determination of MCPD fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization
title_sort novel fast analytical method for indirect determination of mcpd fatty acid esters in edible oils and fats based on simultaneous extraction and derivatization
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486935/
https://www.ncbi.nlm.nih.gov/pubmed/28484809
http://dx.doi.org/10.1007/s00216-017-0381-z
work_keys_str_mv AT jedrkiewiczrenata novelfastanalyticalmethodforindirectdeterminationofmcpdfattyacidestersinedibleoilsandfatsbasedonsimultaneousextractionandderivatization
AT głowaczrozynskaagnieszka novelfastanalyticalmethodforindirectdeterminationofmcpdfattyacidestersinedibleoilsandfatsbasedonsimultaneousextractionandderivatization
AT gromadzkajustyna novelfastanalyticalmethodforindirectdeterminationofmcpdfattyacidestersinedibleoilsandfatsbasedonsimultaneousextractionandderivatization
AT konieczkapiotr novelfastanalyticalmethodforindirectdeterminationofmcpdfattyacidestersinedibleoilsandfatsbasedonsimultaneousextractionandderivatization
AT namiesnikjacek novelfastanalyticalmethodforindirectdeterminationofmcpdfattyacidestersinedibleoilsandfatsbasedonsimultaneousextractionandderivatization