HEK293T cell lines defective for O-linked glycosylation

Here we describe derivatives of the HEK293T cell line that are defective in their ability to generate mucin-type O-linked glycosylation. Using CRISPR/Cas9 and a single-cell GFP-sorting procedure, the UDP-galactose-4-epimerase (GALE), galactokinase 1 (GALK1), and galactokinase 2 (GALK2) genes were kn...

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Detalles Bibliográficos
Autores principales: Termini, James M., Silver, Zachary A., Connor, Bryony, Antonopoulos, Aristotelis, Haslam, Stuart M., Dell, Anne, Desrosiers, Ronald C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487050/
https://www.ncbi.nlm.nih.gov/pubmed/28654657
http://dx.doi.org/10.1371/journal.pone.0179949

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487050/
https://www.ncbi.nlm.nih.gov/pubmed/28654657
http://dx.doi.org/10.1371/journal.pone.0179949

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