Cargando…

Development and Validation of Non simultaneous Retinal Image Acquisition–Based Retinal Oximeter

The purpose of this study was to develop and validate a tool for the measurement of retinal oxygen saturation using a conventional fundus camera–based nonsimultaneous imaging technique. Retinal oximetry setup comprising a conventional Zeiss FF450IR fundus camera, dual wavelength band-pass filters of...

Descripción completa

Detalles Bibliográficos
Autores principales: Singh, Sumeer, Velu, Gunasekaran, Raman, Rajiv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487317/
https://www.ncbi.nlm.nih.gov/pubmed/28655932
http://dx.doi.org/10.1038/s41598-017-04085-x
_version_ 1783246434862104576
author Singh, Sumeer
Velu, Gunasekaran
Raman, Rajiv
author_facet Singh, Sumeer
Velu, Gunasekaran
Raman, Rajiv
author_sort Singh, Sumeer
collection PubMed
description The purpose of this study was to develop and validate a tool for the measurement of retinal oxygen saturation using a conventional fundus camera–based nonsimultaneous imaging technique. Retinal oximetry setup comprising a conventional Zeiss FF450IR fundus camera, dual wavelength band-pass filters of wavelengths 570 and 600 nm were used. Image analysis was performed using MATLAB R2013b. All the study participants underwent comprehensive eye examination, fundus examination, complete hemogram analysis, and evaluation of systemic hemodynamics. Fundus images were captured by a nonsimultaneous retinal oximetry. A total of 45 subjects were included in the analysis. Median age of the subjects was 21 years ranging from 19 to 34 years. The median retinal arteriolar and venular oxygen saturation was 94.7% and 55.8%. Comparison of retinal oxygen saturation between three visits showed no statistically significant difference for both arteriolar (p = 0.33)and venular oxygen saturation (p = 0.79). Intraclass correlation coefficients for test–retest, short-term, and day-to-day repeatability were 0.84, 0.90, and 0.86 for arteriolar oxygen saturation and 0.92, 0.98, and 0.98 for retinal venular oxygen saturation. Oxygen saturation in retinal arteriolar and venular blood vessels can be measured by nonsimultaneous image acquisition technique using a conventional fundus camera with good repeatability.
format Online
Article
Text
id pubmed-5487317
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54873172017-06-30 Development and Validation of Non simultaneous Retinal Image Acquisition–Based Retinal Oximeter Singh, Sumeer Velu, Gunasekaran Raman, Rajiv Sci Rep Article The purpose of this study was to develop and validate a tool for the measurement of retinal oxygen saturation using a conventional fundus camera–based nonsimultaneous imaging technique. Retinal oximetry setup comprising a conventional Zeiss FF450IR fundus camera, dual wavelength band-pass filters of wavelengths 570 and 600 nm were used. Image analysis was performed using MATLAB R2013b. All the study participants underwent comprehensive eye examination, fundus examination, complete hemogram analysis, and evaluation of systemic hemodynamics. Fundus images were captured by a nonsimultaneous retinal oximetry. A total of 45 subjects were included in the analysis. Median age of the subjects was 21 years ranging from 19 to 34 years. The median retinal arteriolar and venular oxygen saturation was 94.7% and 55.8%. Comparison of retinal oxygen saturation between three visits showed no statistically significant difference for both arteriolar (p = 0.33)and venular oxygen saturation (p = 0.79). Intraclass correlation coefficients for test–retest, short-term, and day-to-day repeatability were 0.84, 0.90, and 0.86 for arteriolar oxygen saturation and 0.92, 0.98, and 0.98 for retinal venular oxygen saturation. Oxygen saturation in retinal arteriolar and venular blood vessels can be measured by nonsimultaneous image acquisition technique using a conventional fundus camera with good repeatability. Nature Publishing Group UK 2017-06-27 /pmc/articles/PMC5487317/ /pubmed/28655932 http://dx.doi.org/10.1038/s41598-017-04085-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Singh, Sumeer
Velu, Gunasekaran
Raman, Rajiv
Development and Validation of Non simultaneous Retinal Image Acquisition–Based Retinal Oximeter
title Development and Validation of Non simultaneous Retinal Image Acquisition–Based Retinal Oximeter
title_full Development and Validation of Non simultaneous Retinal Image Acquisition–Based Retinal Oximeter
title_fullStr Development and Validation of Non simultaneous Retinal Image Acquisition–Based Retinal Oximeter
title_full_unstemmed Development and Validation of Non simultaneous Retinal Image Acquisition–Based Retinal Oximeter
title_short Development and Validation of Non simultaneous Retinal Image Acquisition–Based Retinal Oximeter
title_sort development and validation of non simultaneous retinal image acquisition–based retinal oximeter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487317/
https://www.ncbi.nlm.nih.gov/pubmed/28655932
http://dx.doi.org/10.1038/s41598-017-04085-x
work_keys_str_mv AT singhsumeer developmentandvalidationofnonsimultaneousretinalimageacquisitionbasedretinaloximeter
AT velugunasekaran developmentandvalidationofnonsimultaneousretinalimageacquisitionbasedretinaloximeter
AT ramanrajiv developmentandvalidationofnonsimultaneousretinalimageacquisitionbasedretinaloximeter