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Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard

Microbial eukaryotes can play prominent roles in the Arctic marine ecosystem, but their diversity and variability is not well known in the ice-covered ecosystems. We determined the community composition of microbial eukaryotes in an Arctic under-ice spring bloom north of Svalbard using metabarcoding...

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Autores principales: Meshram, Archana R., Vader, Anna, Kristiansen, Svein, Gabrielsen, Tove M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487457/
https://www.ncbi.nlm.nih.gov/pubmed/28702000
http://dx.doi.org/10.3389/fmicb.2017.01099
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author Meshram, Archana R.
Vader, Anna
Kristiansen, Svein
Gabrielsen, Tove M.
author_facet Meshram, Archana R.
Vader, Anna
Kristiansen, Svein
Gabrielsen, Tove M.
author_sort Meshram, Archana R.
collection PubMed
description Microbial eukaryotes can play prominent roles in the Arctic marine ecosystem, but their diversity and variability is not well known in the ice-covered ecosystems. We determined the community composition of microbial eukaryotes in an Arctic under-ice spring bloom north of Svalbard using metabarcoding of DNA and RNA from the hypervariable V4 region of 18S nrDNA. At the two stations studied, the photosynthetic biomass was dominated by protists >3 μm and was concentrated in the upper 70–80 m, above the thermocline and halocline. Hierarchical cluster analyses as well as ordination analyses showed a distinct clustering of the microbial eukaryote communities according to a combination of water mass and local environmental characteristics. While samples collected in the surface mixed layer differed distinctly between the two sites, the deeper communities collected in Atlantic Water were fairly similar despite being geographically distant. The differentiation of the microbial eukaryote communities of the upper mixed water was probably driven by local development and advection, while the lack of such differentiation in the communities of Atlantic Water reflects the homogenizing effect of water currents on microbial communities.
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spelling pubmed-54874572017-07-12 Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard Meshram, Archana R. Vader, Anna Kristiansen, Svein Gabrielsen, Tove M. Front Microbiol Microbiology Microbial eukaryotes can play prominent roles in the Arctic marine ecosystem, but their diversity and variability is not well known in the ice-covered ecosystems. We determined the community composition of microbial eukaryotes in an Arctic under-ice spring bloom north of Svalbard using metabarcoding of DNA and RNA from the hypervariable V4 region of 18S nrDNA. At the two stations studied, the photosynthetic biomass was dominated by protists >3 μm and was concentrated in the upper 70–80 m, above the thermocline and halocline. Hierarchical cluster analyses as well as ordination analyses showed a distinct clustering of the microbial eukaryote communities according to a combination of water mass and local environmental characteristics. While samples collected in the surface mixed layer differed distinctly between the two sites, the deeper communities collected in Atlantic Water were fairly similar despite being geographically distant. The differentiation of the microbial eukaryote communities of the upper mixed water was probably driven by local development and advection, while the lack of such differentiation in the communities of Atlantic Water reflects the homogenizing effect of water currents on microbial communities. Frontiers Media S.A. 2017-06-28 /pmc/articles/PMC5487457/ /pubmed/28702000 http://dx.doi.org/10.3389/fmicb.2017.01099 Text en Copyright © 2017 Meshram, Vader, Kristiansen and Gabrielsen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Meshram, Archana R.
Vader, Anna
Kristiansen, Svein
Gabrielsen, Tove M.
Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard
title Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard
title_full Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard
title_fullStr Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard
title_full_unstemmed Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard
title_short Microbial Eukaryotes in an Arctic Under-Ice Spring Bloom North of Svalbard
title_sort microbial eukaryotes in an arctic under-ice spring bloom north of svalbard
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487457/
https://www.ncbi.nlm.nih.gov/pubmed/28702000
http://dx.doi.org/10.3389/fmicb.2017.01099
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