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Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation

AIM: To investigate the role of heat shock protein (HSP)-glycoprotein (gp)96 in dendritic cells (DCs) and lymphocytes induction in gastric cancer (GC). METHODS: Human GC cell lines KATOIII, MKN-28 and SGC-7901 were infected with adenovirus gp96 at a multiplicity of infection of 100. gp96-GC antigen...

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Autores principales: Lu, Wen-Wen, Zhang, Hong, Li, You-Ming, Ji, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487502/
https://www.ncbi.nlm.nih.gov/pubmed/28706421
http://dx.doi.org/10.3748/wjg.v23.i24.4390
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author Lu, Wen-Wen
Zhang, Hong
Li, You-Ming
Ji, Feng
author_facet Lu, Wen-Wen
Zhang, Hong
Li, You-Ming
Ji, Feng
author_sort Lu, Wen-Wen
collection PubMed
description AIM: To investigate the role of heat shock protein (HSP)-glycoprotein (gp)96 in dendritic cells (DCs) and lymphocytes induction in gastric cancer (GC). METHODS: Human GC cell lines KATOIII, MKN-28 and SGC-7901 were infected with adenovirus gp96 at a multiplicity of infection of 100. gp96-GC antigen peptide complexes were purified. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, lactate dehydrogenase (LDH) release assay and enzyme-linked immunosorbent assay were used to determine allo-reactive T cell stimulation, natural killer (NK) cell activity and expression of cytokines (such as interleukin (IL)-10, IL-12, interferon (IFN)-γ and tumor necrosis factor (TNF)-α), respectively. Effect of cytotoxic T lymphocyte (CTL) on DCs incubated with HSP-gp96 was also evaluated by LDH release. All assays were performed in triplicate and the average values were reported. Comparison between groups was conducted using Student’s t test. RESULTS: T cells incubated with HSP-gp96 exhibited a marked increase in proliferation in a dose-dependent manner (P < 0.05). NK cell activity after gp96-GC peptide complex treatment was significantly higher than that after antigen peptide treatment (P < 0.05). The activity of CTLs incubated with DCs from three GC cells lines was obviously higher than that stimulated by GC antigen at ratios of 50: 1, 25: 1, 10: 1, and 5: 1 (P < 0.05). Furthermore, the secretion of TNF-α, IL-10, IL-12 (P70) and IFN-γ markedly increased after incubation with HSP-gp96 (P < 0.05). CONCLUSION: HSP-gp96 promotes T cell response, enhances DC antigen presentation and induces cytokine secretion, as well. HSP-gp96 has potential as immunotherapy for elimination of residual GC cells.
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spelling pubmed-54875022017-07-13 Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation Lu, Wen-Wen Zhang, Hong Li, You-Ming Ji, Feng World J Gastroenterol Basic Study AIM: To investigate the role of heat shock protein (HSP)-glycoprotein (gp)96 in dendritic cells (DCs) and lymphocytes induction in gastric cancer (GC). METHODS: Human GC cell lines KATOIII, MKN-28 and SGC-7901 were infected with adenovirus gp96 at a multiplicity of infection of 100. gp96-GC antigen peptide complexes were purified. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, lactate dehydrogenase (LDH) release assay and enzyme-linked immunosorbent assay were used to determine allo-reactive T cell stimulation, natural killer (NK) cell activity and expression of cytokines (such as interleukin (IL)-10, IL-12, interferon (IFN)-γ and tumor necrosis factor (TNF)-α), respectively. Effect of cytotoxic T lymphocyte (CTL) on DCs incubated with HSP-gp96 was also evaluated by LDH release. All assays were performed in triplicate and the average values were reported. Comparison between groups was conducted using Student’s t test. RESULTS: T cells incubated with HSP-gp96 exhibited a marked increase in proliferation in a dose-dependent manner (P < 0.05). NK cell activity after gp96-GC peptide complex treatment was significantly higher than that after antigen peptide treatment (P < 0.05). The activity of CTLs incubated with DCs from three GC cells lines was obviously higher than that stimulated by GC antigen at ratios of 50: 1, 25: 1, 10: 1, and 5: 1 (P < 0.05). Furthermore, the secretion of TNF-α, IL-10, IL-12 (P70) and IFN-γ markedly increased after incubation with HSP-gp96 (P < 0.05). CONCLUSION: HSP-gp96 promotes T cell response, enhances DC antigen presentation and induces cytokine secretion, as well. HSP-gp96 has potential as immunotherapy for elimination of residual GC cells. Baishideng Publishing Group Inc 2017-06-28 2017-06-28 /pmc/articles/PMC5487502/ /pubmed/28706421 http://dx.doi.org/10.3748/wjg.v23.i24.4390 Text en ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Basic Study
Lu, Wen-Wen
Zhang, Hong
Li, You-Ming
Ji, Feng
Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation
title Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation
title_full Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation
title_fullStr Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation
title_full_unstemmed Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation
title_short Gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation
title_sort gastric cancer-derived heat shock protein-gp96 peptide complex enhances dendritic cell activation
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487502/
https://www.ncbi.nlm.nih.gov/pubmed/28706421
http://dx.doi.org/10.3748/wjg.v23.i24.4390
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