Rapid diagnosis of equine influenza by highly sensitive silver amplification immunochromatography system

Equine influenza (EI) is a respiratory disease caused by equine influenza A virus (EIV, H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previously reported that some rapid antigen detection (RAD) tests are fit for diagnosing EI although their sensitivity is not optimal. H...

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Detalles Bibliográficos
Autores principales: YAMANAKA, Takashi, NEMOTO, Manabu, BANNAI, Hiroshi, TSUJIMURA, Koji, KONDO, Takashi, MATSUMURA, Tomio, FU, Tao Qi Huang, FERNANDEZ, Charlene Judith, GILDEA, Sarah, CULLINANE, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5487783/
https://www.ncbi.nlm.nih.gov/pubmed/28458275
http://dx.doi.org/10.1292/jvms.17-0105
Descripción
Sumario:Equine influenza (EI) is a respiratory disease caused by equine influenza A virus (EIV, H3N8) infection. Rapid diagnosis is essential to limit the disease spread. We previously reported that some rapid antigen detection (RAD) tests are fit for diagnosing EI although their sensitivity is not optimal. Here, we evaluated the performance of the newly developed RAD test using silver amplification immunochromatography (Quick Chaser Auto Flu A, B: QCA) to diagnose EI. The detection limits of QCA for EIVs were five-fold lower than the conventional RAD tests. The duration of virus antigen detection in the infected horses was longer than the conventional RAD tests. We conclude that QCA could be a valuable diagnostic method for EI.

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