Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context‐Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space

Catalytic promiscuity can facilitate evolution of enzyme functions—a multifunctional catalyst may act as a springboard for efficient functional adaptation. We test the effect of single mutations on multiple activities in two groups of promiscuous AP superfamily members to probe this hypothesis. We q...

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Detalles Bibliográficos
Autores principales: Bayer, Christopher D., van Loo, Bert, Hollfelder, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488252/
https://www.ncbi.nlm.nih.gov/pubmed/28464395
http://dx.doi.org/10.1002/cbic.201600657

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488252/
https://www.ncbi.nlm.nih.gov/pubmed/28464395
http://dx.doi.org/10.1002/cbic.201600657

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