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Ras oncogene and Hypoxia-inducible factor-1 alpha (hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo[a]pyrene.
Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 an...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488454/ https://www.ncbi.nlm.nih.gov/pubmed/28486571 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0066 |
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author | da Silva, Grazyelle Sebrenski Fé, Luciana Mara Lopes da Silva, Maria de Nazaré Paula Val, Vera Maria Fonseca de Almeida e |
author_facet | da Silva, Grazyelle Sebrenski Fé, Luciana Mara Lopes da Silva, Maria de Nazaré Paula Val, Vera Maria Fonseca de Almeida e |
author_sort | da Silva, Grazyelle Sebrenski |
collection | PubMed |
description | Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay. Ras oncogene was overexpressed in fish exposed to 4, 8 of 16 μmol/kg B[a]P, showing 4.96, 7.10 and 6.78-fold increases, respectively. Overexpression also occurred in hif-1α in fish injected with 4 and 8 μmol/kg B[a]P, showing 8.82 and 4.64-fold increases, respectively. Histopathological damage in fish liver was classified as irreparable in fish exposed to 8, 16 and 32 μmol/kg μM B[a]P. The genotoxic damage increased in fish injected with 8 and 16 μmol/kg in comparison with the control group. Acute exposure of B[a]P was capable to interrupt the expression of ras oncogene and hif-1α, and increase DNA breaks due to tissue damage. |
format | Online Article Text |
id | pubmed-5488454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-54884542017-07-11 Ras oncogene and Hypoxia-inducible factor-1 alpha (hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo[a]pyrene. da Silva, Grazyelle Sebrenski Fé, Luciana Mara Lopes da Silva, Maria de Nazaré Paula Val, Vera Maria Fonseca de Almeida e Genet Mol Biol Mutagenesis Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay. Ras oncogene was overexpressed in fish exposed to 4, 8 of 16 μmol/kg B[a]P, showing 4.96, 7.10 and 6.78-fold increases, respectively. Overexpression also occurred in hif-1α in fish injected with 4 and 8 μmol/kg B[a]P, showing 8.82 and 4.64-fold increases, respectively. Histopathological damage in fish liver was classified as irreparable in fish exposed to 8, 16 and 32 μmol/kg μM B[a]P. The genotoxic damage increased in fish injected with 8 and 16 μmol/kg in comparison with the control group. Acute exposure of B[a]P was capable to interrupt the expression of ras oncogene and hif-1α, and increase DNA breaks due to tissue damage. Sociedade Brasileira de Genética 2017-05-08 2017 /pmc/articles/PMC5488454/ /pubmed/28486571 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0066 Text en Copyright © 2017, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited. |
spellingShingle | Mutagenesis da Silva, Grazyelle Sebrenski Fé, Luciana Mara Lopes da Silva, Maria de Nazaré Paula Val, Vera Maria Fonseca de Almeida e Ras oncogene and Hypoxia-inducible factor-1 alpha (hif-1α) expression in the Amazon fish Colossoma macropomum (Cuvier, 1818) exposed to benzo[a]pyrene. |
title |
Ras oncogene and Hypoxia-inducible factor-1 alpha
(hif-1α) expression in the Amazon fish Colossoma
macropomum (Cuvier, 1818) exposed to benzo[a]pyrene. |
title_full |
Ras oncogene and Hypoxia-inducible factor-1 alpha
(hif-1α) expression in the Amazon fish Colossoma
macropomum (Cuvier, 1818) exposed to benzo[a]pyrene. |
title_fullStr |
Ras oncogene and Hypoxia-inducible factor-1 alpha
(hif-1α) expression in the Amazon fish Colossoma
macropomum (Cuvier, 1818) exposed to benzo[a]pyrene. |
title_full_unstemmed |
Ras oncogene and Hypoxia-inducible factor-1 alpha
(hif-1α) expression in the Amazon fish Colossoma
macropomum (Cuvier, 1818) exposed to benzo[a]pyrene. |
title_short |
Ras oncogene and Hypoxia-inducible factor-1 alpha
(hif-1α) expression in the Amazon fish Colossoma
macropomum (Cuvier, 1818) exposed to benzo[a]pyrene. |
title_sort | ras oncogene and hypoxia-inducible factor-1 alpha
(hif-1α) expression in the amazon fish colossoma
macropomum (cuvier, 1818) exposed to benzo[a]pyrene. |
topic | Mutagenesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488454/ https://www.ncbi.nlm.nih.gov/pubmed/28486571 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0066 |
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