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Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women
Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488457/ https://www.ncbi.nlm.nih.gov/pubmed/28497838 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0123 |
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author | de Oliveira, Jéssica Tureck, Luciane Viater dos Santos, Willian Saliba, Louise Farah Schenknecht, Caroline Schovanz Scaraboto, Débora Souza, Ricardo Lehtonen R. Furtado-Alle, Lupe |
author_facet | de Oliveira, Jéssica Tureck, Luciane Viater dos Santos, Willian Saliba, Louise Farah Schenknecht, Caroline Schovanz Scaraboto, Débora Souza, Ricardo Lehtonen R. Furtado-Alle, Lupe |
author_sort | de Oliveira, Jéssica |
collection | PubMed |
description | Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms. |
format | Online Article Text |
id | pubmed-5488457 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-54884572017-07-11 Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women de Oliveira, Jéssica Tureck, Luciane Viater dos Santos, Willian Saliba, Louise Farah Schenknecht, Caroline Schovanz Scaraboto, Débora Souza, Ricardo Lehtonen R. Furtado-Alle, Lupe Genet Mol Biol Human and Medical Genetics Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms. Sociedade Brasileira de Genética 2017-05-11 2017 /pmc/articles/PMC5488457/ /pubmed/28497838 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0123 Text en Copyright © 2017, Sociedade Brasileira de Genética. http://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited. |
spellingShingle | Human and Medical Genetics de Oliveira, Jéssica Tureck, Luciane Viater dos Santos, Willian Saliba, Louise Farah Schenknecht, Caroline Schovanz Scaraboto, Débora Souza, Ricardo Lehtonen R. Furtado-Alle, Lupe Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women |
title | Effect of BCHE single nucleotide polymorphisms on lipid
metabolism markers in women |
title_full | Effect of BCHE single nucleotide polymorphisms on lipid
metabolism markers in women |
title_fullStr | Effect of BCHE single nucleotide polymorphisms on lipid
metabolism markers in women |
title_full_unstemmed | Effect of BCHE single nucleotide polymorphisms on lipid
metabolism markers in women |
title_short | Effect of BCHE single nucleotide polymorphisms on lipid
metabolism markers in women |
title_sort | effect of bche single nucleotide polymorphisms on lipid
metabolism markers in women |
topic | Human and Medical Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488457/ https://www.ncbi.nlm.nih.gov/pubmed/28497838 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0123 |
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