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Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients

BACKGROUND: Helicobacter pylori infection is prevalent in Ghana. The development of gastro-duodenal disease is dependent on virulence of the infecting strain, host susceptibility and environmental factors. Helicobacter pylori cagA and vacA strains induce more inflammation, ulceration and oncogenesis...

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Autores principales: Archampong, Timothy N., Asmah, Richard H., Aidoo, Ebenezer K., Wiredu, Edwin K., Gyasi, Richard K., Adjei, David N., Beleza, Sandra, Bayliss, Christopher D., Krogfelt, Karen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488471/
https://www.ncbi.nlm.nih.gov/pubmed/28655347
http://dx.doi.org/10.1186/s13104-017-2542-8
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author Archampong, Timothy N.
Asmah, Richard H.
Aidoo, Ebenezer K.
Wiredu, Edwin K.
Gyasi, Richard K.
Adjei, David N.
Beleza, Sandra
Bayliss, Christopher D.
Krogfelt, Karen
author_facet Archampong, Timothy N.
Asmah, Richard H.
Aidoo, Ebenezer K.
Wiredu, Edwin K.
Gyasi, Richard K.
Adjei, David N.
Beleza, Sandra
Bayliss, Christopher D.
Krogfelt, Karen
author_sort Archampong, Timothy N.
collection PubMed
description BACKGROUND: Helicobacter pylori infection is prevalent in Ghana. The development of gastro-duodenal disease is dependent on virulence of the infecting strain, host susceptibility and environmental factors. Helicobacter pylori cagA and vacA strains induce more inflammation, ulceration and oncogenesis. Here, for the first time we present data on H. pylori cagA and vacA genes and their association with gastro-duodenal disease in Ghana. A total of 159 patients with dyspepsia at Korle-Bu Teaching Hospital, Accra, were investigated for H. pylori with urease-CLO, of which 113 (71.1%) were positive. Genomic DNA was extracted from antral biopsies using QIAGEN DNeasy kit. Detection of H. pylori vacA and cagA genes were determined by PCR as previously described. RESULTS: In total, 110 (69.2%) vacAs1, 71 (44.7%) vacAm1, 35 (22.0%) vacAm2, 77 (48.4%) cagA-(hydrophilic region) and 109 (68.6%) cagA-(internal duplication region) were detected. In multivariate analysis, duodenal ulcer was more likely than other diagnoses to have detectable cagA-(hydrophilic region) (OR 3.1 CI 1.2–7.9) or vacAs1m1 (OR 6.5 CI 1.2–34.0). CONCLUSIONS: Majority of biopsies were colonized with H. pylori harboring both cagA and vacA. H. pylori cagA-(internal duplication region) was more prevalent than cagA-(hydrophilic region). Duodenal ulcer was more likely than other diagnoses to have detectable cagA-(hydrophilic region) or vacAs1m1.
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spelling pubmed-54884712017-06-30 Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients Archampong, Timothy N. Asmah, Richard H. Aidoo, Ebenezer K. Wiredu, Edwin K. Gyasi, Richard K. Adjei, David N. Beleza, Sandra Bayliss, Christopher D. Krogfelt, Karen BMC Res Notes Research Article BACKGROUND: Helicobacter pylori infection is prevalent in Ghana. The development of gastro-duodenal disease is dependent on virulence of the infecting strain, host susceptibility and environmental factors. Helicobacter pylori cagA and vacA strains induce more inflammation, ulceration and oncogenesis. Here, for the first time we present data on H. pylori cagA and vacA genes and their association with gastro-duodenal disease in Ghana. A total of 159 patients with dyspepsia at Korle-Bu Teaching Hospital, Accra, were investigated for H. pylori with urease-CLO, of which 113 (71.1%) were positive. Genomic DNA was extracted from antral biopsies using QIAGEN DNeasy kit. Detection of H. pylori vacA and cagA genes were determined by PCR as previously described. RESULTS: In total, 110 (69.2%) vacAs1, 71 (44.7%) vacAm1, 35 (22.0%) vacAm2, 77 (48.4%) cagA-(hydrophilic region) and 109 (68.6%) cagA-(internal duplication region) were detected. In multivariate analysis, duodenal ulcer was more likely than other diagnoses to have detectable cagA-(hydrophilic region) (OR 3.1 CI 1.2–7.9) or vacAs1m1 (OR 6.5 CI 1.2–34.0). CONCLUSIONS: Majority of biopsies were colonized with H. pylori harboring both cagA and vacA. H. pylori cagA-(internal duplication region) was more prevalent than cagA-(hydrophilic region). Duodenal ulcer was more likely than other diagnoses to have detectable cagA-(hydrophilic region) or vacAs1m1. BioMed Central 2017-06-27 /pmc/articles/PMC5488471/ /pubmed/28655347 http://dx.doi.org/10.1186/s13104-017-2542-8 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Archampong, Timothy N.
Asmah, Richard H.
Aidoo, Ebenezer K.
Wiredu, Edwin K.
Gyasi, Richard K.
Adjei, David N.
Beleza, Sandra
Bayliss, Christopher D.
Krogfelt, Karen
Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients
title Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients
title_full Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients
title_fullStr Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients
title_full_unstemmed Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients
title_short Helicobacter pylori cagA and vacA genes in dyspeptic Ghanaian patients
title_sort helicobacter pylori caga and vaca genes in dyspeptic ghanaian patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488471/
https://www.ncbi.nlm.nih.gov/pubmed/28655347
http://dx.doi.org/10.1186/s13104-017-2542-8
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