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Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis

Mature adipocytes are the major cell type in adipose tissue. This study aimed to explore the osteogenic potency of dedifferentiated fat cells obtained from osteoporotic patients (opDFATs) in vitro and in vivo. Mature adipocytes and adipose-derived stem cells (opASCs) were harvested from subcutaneous...

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Autores principales: Hao, Wei, Jiang, Chuanqiang, Jiang, Ming, Wang, Tian, Wang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488489/
https://www.ncbi.nlm.nih.gov/pubmed/28672891
http://dx.doi.org/10.3892/etm.2017.4465
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author Hao, Wei
Jiang, Chuanqiang
Jiang, Ming
Wang, Tian
Wang, Xin
author_facet Hao, Wei
Jiang, Chuanqiang
Jiang, Ming
Wang, Tian
Wang, Xin
author_sort Hao, Wei
collection PubMed
description Mature adipocytes are the major cell type in adipose tissue. This study aimed to explore the osteogenic potency of dedifferentiated fat cells obtained from osteoporotic patients (opDFATs) in vitro and in vivo. Mature adipocytes and adipose-derived stem cells (opASCs) were harvested from subcutaneous adipose tissue. Mature adipocytes were dedifferentiated to produce opDFATs by the ceiling culture method. OpDFATs were osteogenically induced in vitro with opASCs as a control. Cell growth, alkaline phosphatase (ALPase) activity and cell mineralization were determined, and expression levels of osteogenesis-specific genes (collagen I, osteocalcin and bone sialoprotein) were analyzed using quantitative reverse transcription polymerase chain reaction. After 14 days, the opDFATs were combined with a poly(lactide-co-glycolide)-β-tricalcium phosphate porous scaffold after being suspended in collagen I gel and implanted into nude mice for 4 weeks prior to histological analysis. Unilocular lipid droplets in mature adipocytes gradually split into smaller droplets and disappeared from the cytoplasm. Mature adipocytes dedifferentiated to opDFATs and cell morphology changed from spherical to elongated. High levels of ALPase and cell mineralization were observed in opDFATs by staining. No significant differences were found between the growth curves, ALPase activity, cell mineralization and expression levels of osteogenesis-specific genes between opDFATS and opASCs. After implantation for 4 weeks, new bone tissue was observed histologically in the opDFATs-based biocomposite. OpDFATs are implicated as a novel type of seed cell for bone tissue engineering based on their osteogenic potency and higher abundance in adipose tissue compared with opASCs.
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spelling pubmed-54884892017-06-30 Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis Hao, Wei Jiang, Chuanqiang Jiang, Ming Wang, Tian Wang, Xin Exp Ther Med Articles Mature adipocytes are the major cell type in adipose tissue. This study aimed to explore the osteogenic potency of dedifferentiated fat cells obtained from osteoporotic patients (opDFATs) in vitro and in vivo. Mature adipocytes and adipose-derived stem cells (opASCs) were harvested from subcutaneous adipose tissue. Mature adipocytes were dedifferentiated to produce opDFATs by the ceiling culture method. OpDFATs were osteogenically induced in vitro with opASCs as a control. Cell growth, alkaline phosphatase (ALPase) activity and cell mineralization were determined, and expression levels of osteogenesis-specific genes (collagen I, osteocalcin and bone sialoprotein) were analyzed using quantitative reverse transcription polymerase chain reaction. After 14 days, the opDFATs were combined with a poly(lactide-co-glycolide)-β-tricalcium phosphate porous scaffold after being suspended in collagen I gel and implanted into nude mice for 4 weeks prior to histological analysis. Unilocular lipid droplets in mature adipocytes gradually split into smaller droplets and disappeared from the cytoplasm. Mature adipocytes dedifferentiated to opDFATs and cell morphology changed from spherical to elongated. High levels of ALPase and cell mineralization were observed in opDFATs by staining. No significant differences were found between the growth curves, ALPase activity, cell mineralization and expression levels of osteogenesis-specific genes between opDFATS and opASCs. After implantation for 4 weeks, new bone tissue was observed histologically in the opDFATs-based biocomposite. OpDFATs are implicated as a novel type of seed cell for bone tissue engineering based on their osteogenic potency and higher abundance in adipose tissue compared with opASCs. D.A. Spandidos 2017-07 2017-05-17 /pmc/articles/PMC5488489/ /pubmed/28672891 http://dx.doi.org/10.3892/etm.2017.4465 Text en Copyright: © Hao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Hao, Wei
Jiang, Chuanqiang
Jiang, Ming
Wang, Tian
Wang, Xin
Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis
title Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis
title_full Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis
title_fullStr Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis
title_full_unstemmed Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis
title_short Osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis
title_sort osteogenic potency of dedifferentiated fat cells isolated from elderly people with osteoporosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488489/
https://www.ncbi.nlm.nih.gov/pubmed/28672891
http://dx.doi.org/10.3892/etm.2017.4465
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