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Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription
The therapeutic effect of Uighur prescription on abnormal Savda in asthma patients was evaluated using plasma proteomics in order to elucidate the biological mechanism and identify potential therapeutic targets of abnormal Savda. In the present study, 40 asthma patients with abnormal Savda including...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488641/ https://www.ncbi.nlm.nih.gov/pubmed/28672924 http://dx.doi.org/10.3892/etm.2017.4500 |
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author | Zhang, Canhua Abudula, Abulizi Awuti, Maliyegu Wang, Huiwu Aihemaiti, Xiaimuxikamaier Tusung, Turghun Sulaiman, Xierzhatijiang Upur, Halmurat |
author_facet | Zhang, Canhua Abudula, Abulizi Awuti, Maliyegu Wang, Huiwu Aihemaiti, Xiaimuxikamaier Tusung, Turghun Sulaiman, Xierzhatijiang Upur, Halmurat |
author_sort | Zhang, Canhua |
collection | PubMed |
description | The therapeutic effect of Uighur prescription on abnormal Savda in asthma patients was evaluated using plasma proteomics in order to elucidate the biological mechanism and identify potential therapeutic targets of abnormal Savda. In the present study, 40 asthma patients with abnormal Savda including abnormal Savda Munziq and Savda Mushil were enrolled and treated with Uighur prescription. The effect of Uighur prescription on protein expression and potential targets was investigated by isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics and bioinformatics analysis. Expression of candidate proteins was verified by an enzyme-linked immunosorbent assay. Following treatment with the Uighur prescription, 22 proteins were differentially expressed in the plasma of patients with asthma and abnormal Savda. The majority of these proteins were localized in intermediate filaments and the cytoskeleton and acted as antioxidant enzymes and binding proteins. Furthermore, they participated in the defense and inflammatory response, and the response to oxidative stress and wound healing. Peroxiredoxin 2 and carboxypeptidase B2 expression was significantly upregulated, whereas S100A7 was considerably downregulated in the whole plasma of patients (all P<0.05) in accordance with the iTRAQ proteomics data. Uighur prescription of abnormal Savda may affect the whole regulatory network of protein expression that is altered following the development of abnormal Savda in patients with asthma. |
format | Online Article Text |
id | pubmed-5488641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-54886412017-06-30 Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription Zhang, Canhua Abudula, Abulizi Awuti, Maliyegu Wang, Huiwu Aihemaiti, Xiaimuxikamaier Tusung, Turghun Sulaiman, Xierzhatijiang Upur, Halmurat Exp Ther Med Articles The therapeutic effect of Uighur prescription on abnormal Savda in asthma patients was evaluated using plasma proteomics in order to elucidate the biological mechanism and identify potential therapeutic targets of abnormal Savda. In the present study, 40 asthma patients with abnormal Savda including abnormal Savda Munziq and Savda Mushil were enrolled and treated with Uighur prescription. The effect of Uighur prescription on protein expression and potential targets was investigated by isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics and bioinformatics analysis. Expression of candidate proteins was verified by an enzyme-linked immunosorbent assay. Following treatment with the Uighur prescription, 22 proteins were differentially expressed in the plasma of patients with asthma and abnormal Savda. The majority of these proteins were localized in intermediate filaments and the cytoskeleton and acted as antioxidant enzymes and binding proteins. Furthermore, they participated in the defense and inflammatory response, and the response to oxidative stress and wound healing. Peroxiredoxin 2 and carboxypeptidase B2 expression was significantly upregulated, whereas S100A7 was considerably downregulated in the whole plasma of patients (all P<0.05) in accordance with the iTRAQ proteomics data. Uighur prescription of abnormal Savda may affect the whole regulatory network of protein expression that is altered following the development of abnormal Savda in patients with asthma. D.A. Spandidos 2017-07 2017-05-23 /pmc/articles/PMC5488641/ /pubmed/28672924 http://dx.doi.org/10.3892/etm.2017.4500 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhang, Canhua Abudula, Abulizi Awuti, Maliyegu Wang, Huiwu Aihemaiti, Xiaimuxikamaier Tusung, Turghun Sulaiman, Xierzhatijiang Upur, Halmurat Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription |
title | Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription |
title_full | Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription |
title_fullStr | Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription |
title_full_unstemmed | Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription |
title_short | Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription |
title_sort | plasma proteins as potential targets of abnormal savda syndrome in asthma patients treated with unique uighur prescription |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488641/ https://www.ncbi.nlm.nih.gov/pubmed/28672924 http://dx.doi.org/10.3892/etm.2017.4500 |
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