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Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm

Bacteria of genus Achromobacter are emerging pathogens in cystic fibrosis (CF) capable of biofilm formation and development of antimicrobial resistance. Evolutionary adaptions in the transition from primary to chronic infection were assessed by transcriptomic analysis of successive isolates of Achro...

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Autores principales: Nielsen, Signe M., Meyer, Rikke L., Nørskov-Lauritsen, Niels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488654/
https://www.ncbi.nlm.nih.gov/pubmed/28534862
http://dx.doi.org/10.3390/pathogens6020020
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author Nielsen, Signe M.
Meyer, Rikke L.
Nørskov-Lauritsen, Niels
author_facet Nielsen, Signe M.
Meyer, Rikke L.
Nørskov-Lauritsen, Niels
author_sort Nielsen, Signe M.
collection PubMed
description Bacteria of genus Achromobacter are emerging pathogens in cystic fibrosis (CF) capable of biofilm formation and development of antimicrobial resistance. Evolutionary adaptions in the transition from primary to chronic infection were assessed by transcriptomic analysis of successive isolates of Achromobacter xylosoxidans from a single CF patient. Several efflux pump systems targeting antimicrobial agents were upregulated during the course of the disease, whereas all genes related to motility were downregulated. Genes annotated to subsystems of sulfur metabolism, protein metabolism and potassium metabolism exhibited the strongest upregulation. K+ channel genes were hyperexpressed, and a putative sulfite oxidase was more than 1500 times upregulated. The transcriptome patterns indicated a pivotal role of sulfur metabolism and electrical signalling in Achromobacter biofilms during late stage CF lung disease.
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spelling pubmed-54886542017-06-30 Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm Nielsen, Signe M. Meyer, Rikke L. Nørskov-Lauritsen, Niels Pathogens Article Bacteria of genus Achromobacter are emerging pathogens in cystic fibrosis (CF) capable of biofilm formation and development of antimicrobial resistance. Evolutionary adaptions in the transition from primary to chronic infection were assessed by transcriptomic analysis of successive isolates of Achromobacter xylosoxidans from a single CF patient. Several efflux pump systems targeting antimicrobial agents were upregulated during the course of the disease, whereas all genes related to motility were downregulated. Genes annotated to subsystems of sulfur metabolism, protein metabolism and potassium metabolism exhibited the strongest upregulation. K+ channel genes were hyperexpressed, and a putative sulfite oxidase was more than 1500 times upregulated. The transcriptome patterns indicated a pivotal role of sulfur metabolism and electrical signalling in Achromobacter biofilms during late stage CF lung disease. MDPI 2017-05-19 /pmc/articles/PMC5488654/ /pubmed/28534862 http://dx.doi.org/10.3390/pathogens6020020 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nielsen, Signe M.
Meyer, Rikke L.
Nørskov-Lauritsen, Niels
Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm
title Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm
title_full Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm
title_fullStr Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm
title_full_unstemmed Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm
title_short Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm
title_sort differences in gene expression profiles between early and late isolates in monospecies achromobacter biofilm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488654/
https://www.ncbi.nlm.nih.gov/pubmed/28534862
http://dx.doi.org/10.3390/pathogens6020020
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