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Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model
The aim of the present study was to evaluate the effects of irreversible electroporation (IRE) on the stomach wall following the direct application of IRE onto the organ surface. IRE ablation was performed in 8 Tibetan mini-pigs, which were randomly assigned into two groups based on their ablated ar...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488745/ https://www.ncbi.nlm.nih.gov/pubmed/28672987 http://dx.doi.org/10.3892/etm.2017.4559 |
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author | Li, Jiannan Zeng, Jianying Chen, Jibing Shi, Jian Luo, Xiaomei Fang, Gang Chai, Wei Zhang, Wenlong Liu, Tongjun Niu, Lizhi |
author_facet | Li, Jiannan Zeng, Jianying Chen, Jibing Shi, Jian Luo, Xiaomei Fang, Gang Chai, Wei Zhang, Wenlong Liu, Tongjun Niu, Lizhi |
author_sort | Li, Jiannan |
collection | PubMed |
description | The aim of the present study was to evaluate the effects of irreversible electroporation (IRE) on the stomach wall following the direct application of IRE onto the organ surface. IRE ablation was performed in 8 Tibetan mini-pigs, which were randomly assigned into two groups based on their ablated areas: Group A, gastric cardia, fundus of stomach, gastric body and group B, lesser gastric curvature, greater gastric curvature, stomach pylorus. Two IRE needles were placed in the space between the stomach wall and the liver (not inserted into the stomach tissue), and three lesions were created in each pig. Serum aminotransferase and white blood cell (WBC) levels were measured. Gastroscopy and endoscopic ultrasonography were performed. From each group, 2 pigs were sacrificed on day 7 post-IRE; the remaining pigs were sacrificed on day 28 post-IRE. There were no signs of perforation on the stomach wall. Serum aminotransferase and WBC levels increased in both groups on day 1 post-IRE and decreased gradually thereafter. The gastroscopy procedure revealed oval ulcers on day 7 post-IRE and smaller ulcers on day 28 post-IRE. Transmural necrosis, inflammation and fibrosis were observed at 7 days post-IRE. Healing ulcers were observed at 28 days post-IRE. In conclusion, IRE ablation caused damage to the stomach wall; however, IRE did not induce any perforation. |
format | Online Article Text |
id | pubmed-5488745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-54887452017-06-30 Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model Li, Jiannan Zeng, Jianying Chen, Jibing Shi, Jian Luo, Xiaomei Fang, Gang Chai, Wei Zhang, Wenlong Liu, Tongjun Niu, Lizhi Exp Ther Med Articles The aim of the present study was to evaluate the effects of irreversible electroporation (IRE) on the stomach wall following the direct application of IRE onto the organ surface. IRE ablation was performed in 8 Tibetan mini-pigs, which were randomly assigned into two groups based on their ablated areas: Group A, gastric cardia, fundus of stomach, gastric body and group B, lesser gastric curvature, greater gastric curvature, stomach pylorus. Two IRE needles were placed in the space between the stomach wall and the liver (not inserted into the stomach tissue), and three lesions were created in each pig. Serum aminotransferase and white blood cell (WBC) levels were measured. Gastroscopy and endoscopic ultrasonography were performed. From each group, 2 pigs were sacrificed on day 7 post-IRE; the remaining pigs were sacrificed on day 28 post-IRE. There were no signs of perforation on the stomach wall. Serum aminotransferase and WBC levels increased in both groups on day 1 post-IRE and decreased gradually thereafter. The gastroscopy procedure revealed oval ulcers on day 7 post-IRE and smaller ulcers on day 28 post-IRE. Transmural necrosis, inflammation and fibrosis were observed at 7 days post-IRE. Healing ulcers were observed at 28 days post-IRE. In conclusion, IRE ablation caused damage to the stomach wall; however, IRE did not induce any perforation. D.A. Spandidos 2017-07 2017-06-07 /pmc/articles/PMC5488745/ /pubmed/28672987 http://dx.doi.org/10.3892/etm.2017.4559 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Jiannan Zeng, Jianying Chen, Jibing Shi, Jian Luo, Xiaomei Fang, Gang Chai, Wei Zhang, Wenlong Liu, Tongjun Niu, Lizhi Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model |
title | Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model |
title_full | Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model |
title_fullStr | Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model |
title_full_unstemmed | Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model |
title_short | Evaluation of the safety of irreversible electroporation on the stomach wall using a pig model |
title_sort | evaluation of the safety of irreversible electroporation on the stomach wall using a pig model |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488745/ https://www.ncbi.nlm.nih.gov/pubmed/28672987 http://dx.doi.org/10.3892/etm.2017.4559 |
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