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Lineage-specific Expression of miR-200 Family in Human Embryonic Stem Cells during In Vitro Differentiation

Although microRNAs have emerged as key regulators in diverse cellular processes, the roles of microRNAs are poorly understood in human embryonic stem cells (hESCs) during differentiation into specialized cell types. In this study, we used a microRNA array with 799 human microRNA probes to examine th...

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Autores principales: Kim, Yeji, Kim, Nury, Park, Sang-Wook, Kim, Hyemin, Park, Han-Jin, Han, Yong-Mahn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Stem Cell Research 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488774/
https://www.ncbi.nlm.nih.gov/pubmed/28531914
http://dx.doi.org/10.15283/ijsc17013
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author Kim, Yeji
Kim, Nury
Park, Sang-Wook
Kim, Hyemin
Park, Han-Jin
Han, Yong-Mahn
author_facet Kim, Yeji
Kim, Nury
Park, Sang-Wook
Kim, Hyemin
Park, Han-Jin
Han, Yong-Mahn
author_sort Kim, Yeji
collection PubMed
description Although microRNAs have emerged as key regulators in diverse cellular processes, the roles of microRNAs are poorly understood in human embryonic stem cells (hESCs) during differentiation into specialized cell types. In this study, we used a microRNA array with 799 human microRNA probes to examine the expression profiles of microRNAs in hESCs during differentiation into endodermal and mesodermal lineages in vitro. Among the microRNAs analyzed, 7 and 20 microRNAs were enriched in the developmental process of hESCs into mesodermal and endodermal lineages, respectively. In particular, the expression levels of miR-200 family, which is known to regulate the epithelial to mesenchymal transition (EMT), gradually increased in hESCs during differentiation into hepatocytes while they gradually decreased during differentiation into vascular endothelial cells. Downregulation of ZEB1, a direct target of miR-200 family, and E-CADHERIN, a target protein of ZEB1, was observed in hESCs during differentiation into endodermal and mesodermal lineages, respectively. These results indicate that miR-200 family has an important role in determining the cell fate between endodermal and mesodermal lineages from the pluripotent state.
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spelling pubmed-54887742017-07-07 Lineage-specific Expression of miR-200 Family in Human Embryonic Stem Cells during In Vitro Differentiation Kim, Yeji Kim, Nury Park, Sang-Wook Kim, Hyemin Park, Han-Jin Han, Yong-Mahn Int J Stem Cells Original Article Although microRNAs have emerged as key regulators in diverse cellular processes, the roles of microRNAs are poorly understood in human embryonic stem cells (hESCs) during differentiation into specialized cell types. In this study, we used a microRNA array with 799 human microRNA probes to examine the expression profiles of microRNAs in hESCs during differentiation into endodermal and mesodermal lineages in vitro. Among the microRNAs analyzed, 7 and 20 microRNAs were enriched in the developmental process of hESCs into mesodermal and endodermal lineages, respectively. In particular, the expression levels of miR-200 family, which is known to regulate the epithelial to mesenchymal transition (EMT), gradually increased in hESCs during differentiation into hepatocytes while they gradually decreased during differentiation into vascular endothelial cells. Downregulation of ZEB1, a direct target of miR-200 family, and E-CADHERIN, a target protein of ZEB1, was observed in hESCs during differentiation into endodermal and mesodermal lineages, respectively. These results indicate that miR-200 family has an important role in determining the cell fate between endodermal and mesodermal lineages from the pluripotent state. Korean Society for Stem Cell Research 2017-05 /pmc/articles/PMC5488774/ /pubmed/28531914 http://dx.doi.org/10.15283/ijsc17013 Text en Copyright ©2017, Korean Society for Stem Cell Research This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Yeji
Kim, Nury
Park, Sang-Wook
Kim, Hyemin
Park, Han-Jin
Han, Yong-Mahn
Lineage-specific Expression of miR-200 Family in Human Embryonic Stem Cells during In Vitro Differentiation
title Lineage-specific Expression of miR-200 Family in Human Embryonic Stem Cells during In Vitro Differentiation
title_full Lineage-specific Expression of miR-200 Family in Human Embryonic Stem Cells during In Vitro Differentiation
title_fullStr Lineage-specific Expression of miR-200 Family in Human Embryonic Stem Cells during In Vitro Differentiation
title_full_unstemmed Lineage-specific Expression of miR-200 Family in Human Embryonic Stem Cells during In Vitro Differentiation
title_short Lineage-specific Expression of miR-200 Family in Human Embryonic Stem Cells during In Vitro Differentiation
title_sort lineage-specific expression of mir-200 family in human embryonic stem cells during in vitro differentiation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5488774/
https://www.ncbi.nlm.nih.gov/pubmed/28531914
http://dx.doi.org/10.15283/ijsc17013
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