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Structural mechanism of ATP-independent transcription initiation by RNA polymerase I

Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Saccharomyces cerevisiae Pol I-CF-DNA to 3.8 Å resolution using single-particle cryo-electro...

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Autores principales: Han, Yan, Yan, Chunli, Nguyen, Thi Hoang Duong, Jackobel, Ashleigh J, Ivanov, Ivaylo, Knutson, Bruce A, He, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489313/
https://www.ncbi.nlm.nih.gov/pubmed/28623663
http://dx.doi.org/10.7554/eLife.27414
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author Han, Yan
Yan, Chunli
Nguyen, Thi Hoang Duong
Jackobel, Ashleigh J
Ivanov, Ivaylo
Knutson, Bruce A
He, Yuan
author_facet Han, Yan
Yan, Chunli
Nguyen, Thi Hoang Duong
Jackobel, Ashleigh J
Ivanov, Ivaylo
Knutson, Bruce A
He, Yuan
author_sort Han, Yan
collection PubMed
description Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Saccharomyces cerevisiae Pol I-CF-DNA to 3.8 Å resolution using single-particle cryo-electron microscopy. The structure reveals a bipartite architecture of Core Factor and its recognition of the promoter from −27 to −16. Core Factor’s intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location. Comparison of the three states in this study with the Pol II system suggests that a ratchet motion of the Core Factor-DNA sub-complex at upstream facilitates promoter melting in an ATP-independent manner, distinct from a DNA translocase actively threading the downstream DNA in the Pol II PIC. DOI: http://dx.doi.org/10.7554/eLife.27414.001
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spelling pubmed-54893132017-06-30 Structural mechanism of ATP-independent transcription initiation by RNA polymerase I Han, Yan Yan, Chunli Nguyen, Thi Hoang Duong Jackobel, Ashleigh J Ivanov, Ivaylo Knutson, Bruce A He, Yuan eLife Biophysics and Structural Biology Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Saccharomyces cerevisiae Pol I-CF-DNA to 3.8 Å resolution using single-particle cryo-electron microscopy. The structure reveals a bipartite architecture of Core Factor and its recognition of the promoter from −27 to −16. Core Factor’s intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location. Comparison of the three states in this study with the Pol II system suggests that a ratchet motion of the Core Factor-DNA sub-complex at upstream facilitates promoter melting in an ATP-independent manner, distinct from a DNA translocase actively threading the downstream DNA in the Pol II PIC. DOI: http://dx.doi.org/10.7554/eLife.27414.001 eLife Sciences Publications, Ltd 2017-06-17 /pmc/articles/PMC5489313/ /pubmed/28623663 http://dx.doi.org/10.7554/eLife.27414 Text en © 2017, Han et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biophysics and Structural Biology
Han, Yan
Yan, Chunli
Nguyen, Thi Hoang Duong
Jackobel, Ashleigh J
Ivanov, Ivaylo
Knutson, Bruce A
He, Yuan
Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
title Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
title_full Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
title_fullStr Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
title_full_unstemmed Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
title_short Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
title_sort structural mechanism of atp-independent transcription initiation by rna polymerase i
topic Biophysics and Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489313/
https://www.ncbi.nlm.nih.gov/pubmed/28623663
http://dx.doi.org/10.7554/eLife.27414
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