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Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells

Research into adipose tissue-derived mesenchymal stem cells (AD-MSCs) has demonstrated the feasibility of their use in clinical applications due to their ease of isolation and abundance in adipose tissue. We isolated AD-MSCs from young and old dogs, and the cells were subjected to sequential sub-pas...

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Autores principales: Lee, Jienny, Lee, Keum Sil, Kim, Chan-Lan, Byeon, Jeong Su, Gu, Na-Yeon, Cho, In-Soo, Cha, Sang-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489460/
https://www.ncbi.nlm.nih.gov/pubmed/27456768
http://dx.doi.org/10.4142/jvs.2017.18.2.141
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author Lee, Jienny
Lee, Keum Sil
Kim, Chan-Lan
Byeon, Jeong Su
Gu, Na-Yeon
Cho, In-Soo
Cha, Sang-Ho
author_facet Lee, Jienny
Lee, Keum Sil
Kim, Chan-Lan
Byeon, Jeong Su
Gu, Na-Yeon
Cho, In-Soo
Cha, Sang-Ho
author_sort Lee, Jienny
collection PubMed
description Research into adipose tissue-derived mesenchymal stem cells (AD-MSCs) has demonstrated the feasibility of their use in clinical applications due to their ease of isolation and abundance in adipose tissue. We isolated AD-MSCs from young and old dogs, and the cells were subjected to sequential sub-passaging from passage 1 (P1) to P7. Canine AD-MSCs (cAD-MSCs) were examined for proliferation kinetics, expression of molecules associated with self-renewal, expression of cell surface markers, and differentiation potentials at P3. Cumulative population doubling level was significantly higher in cAD-MSCs of young donors than in those of old donors. In addition, expressions of CD73, CD80, Oct3/4, Nanog, cell survival genes and differentiation potentials were significantly higher in young donors than in old donors. The present study suggests that donor age should be considered when developing cell-based therapies for clinical application of cAD-MSCs.
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spelling pubmed-54894602017-07-05 Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells Lee, Jienny Lee, Keum Sil Kim, Chan-Lan Byeon, Jeong Su Gu, Na-Yeon Cho, In-Soo Cha, Sang-Ho J Vet Sci Original Article Research into adipose tissue-derived mesenchymal stem cells (AD-MSCs) has demonstrated the feasibility of their use in clinical applications due to their ease of isolation and abundance in adipose tissue. We isolated AD-MSCs from young and old dogs, and the cells were subjected to sequential sub-passaging from passage 1 (P1) to P7. Canine AD-MSCs (cAD-MSCs) were examined for proliferation kinetics, expression of molecules associated with self-renewal, expression of cell surface markers, and differentiation potentials at P3. Cumulative population doubling level was significantly higher in cAD-MSCs of young donors than in those of old donors. In addition, expressions of CD73, CD80, Oct3/4, Nanog, cell survival genes and differentiation potentials were significantly higher in young donors than in old donors. The present study suggests that donor age should be considered when developing cell-based therapies for clinical application of cAD-MSCs. The Korean Society of Veterinary Science 2017-06 2017-06-22 /pmc/articles/PMC5489460/ /pubmed/27456768 http://dx.doi.org/10.4142/jvs.2017.18.2.141 Text en © 2017 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lee, Jienny
Lee, Keum Sil
Kim, Chan-Lan
Byeon, Jeong Su
Gu, Na-Yeon
Cho, In-Soo
Cha, Sang-Ho
Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
title Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
title_full Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
title_fullStr Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
title_full_unstemmed Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
title_short Effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
title_sort effect of donor age on the proliferation and multipotency of canine adipose-derived mesenchymal stem cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489460/
https://www.ncbi.nlm.nih.gov/pubmed/27456768
http://dx.doi.org/10.4142/jvs.2017.18.2.141
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