Cargando…
Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 P...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489473/ https://www.ncbi.nlm.nih.gov/pubmed/27515271 http://dx.doi.org/10.4142/jvs.2017.18.2.253 |
_version_ | 1783246792603729920 |
---|---|
author | Kim, Hye Kwon Lyoo, Kwang Soo Huynh, Thi My Le Moon, Hyoung Joon Nguyen, Van Giap Park, Bong Kyun |
author_facet | Kim, Hye Kwon Lyoo, Kwang Soo Huynh, Thi My Le Moon, Hyoung Joon Nguyen, Van Giap Park, Bong Kyun |
author_sort | Kim, Hye Kwon |
collection | PubMed |
description | There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex nested RT-PCR were 10(1.5) TCID(50)/mL for type 2 PRRSV and 10(2) infected cells/mL for PCV2. The kappa statistic, which measures agreement between methods, was 0.867, indicating a good level of agreement. This RNA-based duplex RT-PCR approach can be another way to detect type 2 PRRSV and PCV2 simultaneously and with improved convenience. |
format | Online Article Text |
id | pubmed-5489473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54894732017-07-05 Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples Kim, Hye Kwon Lyoo, Kwang Soo Huynh, Thi My Le Moon, Hyoung Joon Nguyen, Van Giap Park, Bong Kyun J Vet Sci Short Communication There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex nested RT-PCR were 10(1.5) TCID(50)/mL for type 2 PRRSV and 10(2) infected cells/mL for PCV2. The kappa statistic, which measures agreement between methods, was 0.867, indicating a good level of agreement. This RNA-based duplex RT-PCR approach can be another way to detect type 2 PRRSV and PCV2 simultaneously and with improved convenience. The Korean Society of Veterinary Science 2017-06 2017-06-22 /pmc/articles/PMC5489473/ /pubmed/27515271 http://dx.doi.org/10.4142/jvs.2017.18.2.253 Text en © 2017 The Korean Society of Veterinary Science http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Kim, Hye Kwon Lyoo, Kwang Soo Huynh, Thi My Le Moon, Hyoung Joon Nguyen, Van Giap Park, Bong Kyun Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples |
title | Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples |
title_full | Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples |
title_fullStr | Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples |
title_full_unstemmed | Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples |
title_short | Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples |
title_sort | duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489473/ https://www.ncbi.nlm.nih.gov/pubmed/27515271 http://dx.doi.org/10.4142/jvs.2017.18.2.253 |
work_keys_str_mv | AT kimhyekwon duplexnestedreversetranscriptasepolymerasechainreactionforsimultaneousdetectionoftype2porcinereproductiveandrespiratorysyndromevirusandporcinecircovirustype2fromtissuesamples AT lyookwangsoo duplexnestedreversetranscriptasepolymerasechainreactionforsimultaneousdetectionoftype2porcinereproductiveandrespiratorysyndromevirusandporcinecircovirustype2fromtissuesamples AT huynhthimyle duplexnestedreversetranscriptasepolymerasechainreactionforsimultaneousdetectionoftype2porcinereproductiveandrespiratorysyndromevirusandporcinecircovirustype2fromtissuesamples AT moonhyoungjoon duplexnestedreversetranscriptasepolymerasechainreactionforsimultaneousdetectionoftype2porcinereproductiveandrespiratorysyndromevirusandporcinecircovirustype2fromtissuesamples AT nguyenvangiap duplexnestedreversetranscriptasepolymerasechainreactionforsimultaneousdetectionoftype2porcinereproductiveandrespiratorysyndromevirusandporcinecircovirustype2fromtissuesamples AT parkbongkyun duplexnestedreversetranscriptasepolymerasechainreactionforsimultaneousdetectionoftype2porcinereproductiveandrespiratorysyndromevirusandporcinecircovirustype2fromtissuesamples |