ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling

Hepatocellular carcinoma (HCC) is one of the most common cancers and the second leading cause of cancer related death worldwide. ZNF545 is located in the chromosome 19q13.13, which is frequent loss of heterozygosity in human astrocytoma. Methylation of ZNF545 was found frequently in a few kinds of c...

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Detalles Bibliográficos
Autores principales: Yang, Weili, Yang, Shuai, Zhang, Meiying, Gao, Dan, He, Tao, Guo, Mingzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489650/
https://www.ncbi.nlm.nih.gov/pubmed/28680537
http://dx.doi.org/10.18632/genesandcancer.137
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author Yang, Weili
Yang, Shuai
Zhang, Meiying
Gao, Dan
He, Tao
Guo, Mingzhou
author_facet Yang, Weili
Yang, Shuai
Zhang, Meiying
Gao, Dan
He, Tao
Guo, Mingzhou
author_sort Yang, Weili
collection PubMed
description Hepatocellular carcinoma (HCC) is one of the most common cancers and the second leading cause of cancer related death worldwide. ZNF545 is located in the chromosome 19q13.13, which is frequent loss of heterozygosity in human astrocytoma. Methylation of ZNF545 was found frequently in a few kinds of cancers. While the function of ZNF545 in human HCC remains unclear. The purpose of this study is to explore the function and mechanism of ZNF545 in human HCC. Restoration of ZNF545 expression suppressed cell proliferation, migration and invasion, induced G1/S arrest and apoptosis in SNU449 and Huh7 cells. Further study suggested that ZNF545 suppressed HCC cell growth by inhibiting NF-kB signaling. These results were further validated by siRNA knocking down technique in ZNF545 highly expressed HXBF344 cells. In vivo, ZNF545 suppressed tumor growth in SNU449 cell xenograft mice. In conclusion, ZNF545 suppresses human HCC growth by inhibiting NF-kB signaling.
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spelling pubmed-54896502017-07-05 ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling Yang, Weili Yang, Shuai Zhang, Meiying Gao, Dan He, Tao Guo, Mingzhou Genes Cancer Research Paper Hepatocellular carcinoma (HCC) is one of the most common cancers and the second leading cause of cancer related death worldwide. ZNF545 is located in the chromosome 19q13.13, which is frequent loss of heterozygosity in human astrocytoma. Methylation of ZNF545 was found frequently in a few kinds of cancers. While the function of ZNF545 in human HCC remains unclear. The purpose of this study is to explore the function and mechanism of ZNF545 in human HCC. Restoration of ZNF545 expression suppressed cell proliferation, migration and invasion, induced G1/S arrest and apoptosis in SNU449 and Huh7 cells. Further study suggested that ZNF545 suppressed HCC cell growth by inhibiting NF-kB signaling. These results were further validated by siRNA knocking down technique in ZNF545 highly expressed HXBF344 cells. In vivo, ZNF545 suppressed tumor growth in SNU449 cell xenograft mice. In conclusion, ZNF545 suppresses human HCC growth by inhibiting NF-kB signaling. Impact Journals LLC 2017-03 /pmc/articles/PMC5489650/ /pubmed/28680537 http://dx.doi.org/10.18632/genesandcancer.137 Text en Copyright: © 2017 Yang et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Yang, Weili
Yang, Shuai
Zhang, Meiying
Gao, Dan
He, Tao
Guo, Mingzhou
ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling
title ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling
title_full ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling
title_fullStr ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling
title_full_unstemmed ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling
title_short ZNF545 suppresses human hepatocellular carcinoma growth by inhibiting NF-kB signaling
title_sort znf545 suppresses human hepatocellular carcinoma growth by inhibiting nf-kb signaling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489650/
https://www.ncbi.nlm.nih.gov/pubmed/28680537
http://dx.doi.org/10.18632/genesandcancer.137
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