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Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars

In the cut flower market, traditional breeding is still the best way to achieve new rose cultivars. The geographical delocalization of cultivar constitution (generally made in Europe and North America) and plant cultivation (large areas in Africa and South America) represents a limit point for cross...

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Autores principales: Giovannini, Annalisa, Macovei, Anca, Caser, Matteo, Mansuino, Andrea, Ghione, Gian Guido, Savona, Marco, Carbonera, Daniela, Scariot, Valentina, Balestrazzi, Alma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489789/
https://www.ncbi.nlm.nih.gov/pubmed/28441780
http://dx.doi.org/10.3390/plants6020017
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author Giovannini, Annalisa
Macovei, Anca
Caser, Matteo
Mansuino, Andrea
Ghione, Gian Guido
Savona, Marco
Carbonera, Daniela
Scariot, Valentina
Balestrazzi, Alma
author_facet Giovannini, Annalisa
Macovei, Anca
Caser, Matteo
Mansuino, Andrea
Ghione, Gian Guido
Savona, Marco
Carbonera, Daniela
Scariot, Valentina
Balestrazzi, Alma
author_sort Giovannini, Annalisa
collection PubMed
description In the cut flower market, traditional breeding is still the best way to achieve new rose cultivars. The geographical delocalization of cultivar constitution (generally made in Europe and North America) and plant cultivation (large areas in Africa and South America) represents a limit point for crossing and selection. Rose breeders often need to overcome geographical distances, resulting in asynchrony in flowering among crossing parents, by storing and sending pollen. Hence, a key aspect in breeding programs is linked to pollen availability and conservation, jointly with the identification of parameters related to pollen fertility. In this study we present the results of three different trials. In the first, pollen diameter and pollen viability were chosen as fertility predictors of 10 Rosa hybrida commercial cultivars. In the second trial, aliquots of dried pollen grains of six R. hybrida cultivar were stored under two different temperatures (freezer at T = −20 °C and deep freezer at T = −80 °C) and after a wide range of conservation period, their viability was measured. In the third trial, the effective fertilization capacity of frozen pollen of 19 pollen donor cultivars was evaluated during 2015 crossing breeding plan, performing 44 hybridizations and correlating the number of seeds and the ratio seeds/crossing, obtained by each cultivar, with in vitro pollen germination ability.
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spelling pubmed-54897892017-06-30 Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars Giovannini, Annalisa Macovei, Anca Caser, Matteo Mansuino, Andrea Ghione, Gian Guido Savona, Marco Carbonera, Daniela Scariot, Valentina Balestrazzi, Alma Plants (Basel) Article In the cut flower market, traditional breeding is still the best way to achieve new rose cultivars. The geographical delocalization of cultivar constitution (generally made in Europe and North America) and plant cultivation (large areas in Africa and South America) represents a limit point for crossing and selection. Rose breeders often need to overcome geographical distances, resulting in asynchrony in flowering among crossing parents, by storing and sending pollen. Hence, a key aspect in breeding programs is linked to pollen availability and conservation, jointly with the identification of parameters related to pollen fertility. In this study we present the results of three different trials. In the first, pollen diameter and pollen viability were chosen as fertility predictors of 10 Rosa hybrida commercial cultivars. In the second trial, aliquots of dried pollen grains of six R. hybrida cultivar were stored under two different temperatures (freezer at T = −20 °C and deep freezer at T = −80 °C) and after a wide range of conservation period, their viability was measured. In the third trial, the effective fertilization capacity of frozen pollen of 19 pollen donor cultivars was evaluated during 2015 crossing breeding plan, performing 44 hybridizations and correlating the number of seeds and the ratio seeds/crossing, obtained by each cultivar, with in vitro pollen germination ability. MDPI 2017-04-24 /pmc/articles/PMC5489789/ /pubmed/28441780 http://dx.doi.org/10.3390/plants6020017 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giovannini, Annalisa
Macovei, Anca
Caser, Matteo
Mansuino, Andrea
Ghione, Gian Guido
Savona, Marco
Carbonera, Daniela
Scariot, Valentina
Balestrazzi, Alma
Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars
title Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars
title_full Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars
title_fullStr Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars
title_full_unstemmed Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars
title_short Pollen Grain Preservation and Fertility in Valuable Commercial Rose Cultivars
title_sort pollen grain preservation and fertility in valuable commercial rose cultivars
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489789/
https://www.ncbi.nlm.nih.gov/pubmed/28441780
http://dx.doi.org/10.3390/plants6020017
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