Attenuation of Drought Stress in Brassica Seedlings with Exogenous Application of Ca(2+) and H(2)O(2)

Drought is one of the most common abiotic stresses, affecting the growth and productivity of crop plants globally, particularly in arid and semi-arid regions. Different strategies are used to mitigate the impact of drought among crop plants. Exogenous application of different substances are known to decrease the effects of various abiotic stresses, including drought stress. The aim of this study was to evaluate the effect of Ca(2+) and H(2)O(2) in developing drought stress tolerance in Brassica napus “Bulbul-98” seedlings. Brassica napus “Bulbul-98” seedlings were exposed to 5, 10 and 15 mM Ca(2+) and 2, 5 and 10 μM H(2)O(2) concentrations twice at an interval of two days for up to 20 days after germination. Drought stress decreased relative water content (RWC), chlorophyll content and increased proline, H(2)O(2), soluble protein and electrolyte leakage in Brassica seedlings. Exogenous Ca(2+) (5, 10,15 mM) and H(2)O(2) (2, 5, 10 μM) supplementations, during drought stress induction, showed a significant increase in RWC by 5.4%, 18.06%, 26.2% and 6.87%, 13.9%, 18.3% respectively. Similarly, with the exogenous application of Ca(2+) (5, 10, 15 mM) and H(2)O(2) (2, 5, 10 μM), chlorophyll content was increased by 15.03%, 22.2%, and 28.4%, and 9.6%, 23.3%, and 27.5% respectively. It was confirmed that the seedlings under drought stress that were supplemented with Ca(2+) and H(2)O(2) recovered from water content reduction and chlorosis, and were able to grow normally.