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Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light

Comparative analyses of phenotypic and molecular traits of Arabidopsis thaliana grown under standardised conditions is still a challenge using climatic devices supplied with common light sources. These are in most cases fluorescent lights, which have several disadvantages such as heat production at...

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Autores principales: Seiler, Franka, Soll, Jürgen, Bölter, Bettina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489796/
https://www.ncbi.nlm.nih.gov/pubmed/28608805
http://dx.doi.org/10.3390/plants6020024
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author Seiler, Franka
Soll, Jürgen
Bölter, Bettina
author_facet Seiler, Franka
Soll, Jürgen
Bölter, Bettina
author_sort Seiler, Franka
collection PubMed
description Comparative analyses of phenotypic and molecular traits of Arabidopsis thaliana grown under standardised conditions is still a challenge using climatic devices supplied with common light sources. These are in most cases fluorescent lights, which have several disadvantages such as heat production at higher light intensities, an invariable spectral output, and relatively rapid “ageing”. This results in non-desired variations of growth conditions and lowers the comparability of data acquired over extended time periods. In this study, we investigated the growth behaviour of Arabidopsis Col0 under different light conditions, applying fluorescent compared to LED lamps, and we conducted physiological as well as gene expression analyses. By changing the spectral composition and/or light intensity of LEDs we can clearly influence the growth behaviour of Arabidopsis and thereby study phenotypic attributes under very specific light conditions that are stable and reproducible, which is not necessarily given for fluorescent lamps. By using LED lights, we can also roughly mimic the sun light emission spectrum, enabling us to study plant growth in a more natural-like light set-up. We observed distinct growth behaviour under the different light regimes which was reflected by physiological properties of the plants. In conclusion, LEDs provide variable emission spectra for studying plant growth under defined, stable light conditions.
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spelling pubmed-54897962017-06-30 Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light Seiler, Franka Soll, Jürgen Bölter, Bettina Plants (Basel) Article Comparative analyses of phenotypic and molecular traits of Arabidopsis thaliana grown under standardised conditions is still a challenge using climatic devices supplied with common light sources. These are in most cases fluorescent lights, which have several disadvantages such as heat production at higher light intensities, an invariable spectral output, and relatively rapid “ageing”. This results in non-desired variations of growth conditions and lowers the comparability of data acquired over extended time periods. In this study, we investigated the growth behaviour of Arabidopsis Col0 under different light conditions, applying fluorescent compared to LED lamps, and we conducted physiological as well as gene expression analyses. By changing the spectral composition and/or light intensity of LEDs we can clearly influence the growth behaviour of Arabidopsis and thereby study phenotypic attributes under very specific light conditions that are stable and reproducible, which is not necessarily given for fluorescent lamps. By using LED lights, we can also roughly mimic the sun light emission spectrum, enabling us to study plant growth in a more natural-like light set-up. We observed distinct growth behaviour under the different light regimes which was reflected by physiological properties of the plants. In conclusion, LEDs provide variable emission spectra for studying plant growth under defined, stable light conditions. MDPI 2017-06-13 /pmc/articles/PMC5489796/ /pubmed/28608805 http://dx.doi.org/10.3390/plants6020024 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seiler, Franka
Soll, Jürgen
Bölter, Bettina
Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light
title Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light
title_full Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light
title_fullStr Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light
title_full_unstemmed Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light
title_short Comparative Phenotypical and Molecular Analyses of Arabidopsis Grown under Fluorescent and LED Light
title_sort comparative phenotypical and molecular analyses of arabidopsis grown under fluorescent and led light
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5489796/
https://www.ncbi.nlm.nih.gov/pubmed/28608805
http://dx.doi.org/10.3390/plants6020024
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