Cargando…
Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions
BACKGROUND: Anaphylactoid reactions induced by preparations containing red ginseng have been reported. The aim of this study is to evaluate the allergenicity and screen potential allergens in red ginseng extract thoroughly. METHODS: Red ginseng extract (RGE) and different fractions of RGE were prepa...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490175/ https://www.ncbi.nlm.nih.gov/pubmed/28659175 http://dx.doi.org/10.1186/s12967-017-1249-x |
_version_ | 1783246933863694336 |
---|---|
author | Wang, Lu Zhao, Yazheng Yang, Ye Hu, Yuanjia Zou, Xiaohan Yu, Boyang Qi, Jin |
author_facet | Wang, Lu Zhao, Yazheng Yang, Ye Hu, Yuanjia Zou, Xiaohan Yu, Boyang Qi, Jin |
author_sort | Wang, Lu |
collection | PubMed |
description | BACKGROUND: Anaphylactoid reactions induced by preparations containing red ginseng have been reported. The aim of this study is to evaluate the allergenicity and screen potential allergens in red ginseng extract thoroughly. METHODS: Red ginseng extract (RGE) and different fractions of RGE were prepared and evaluated by measuring the degranulation and viability of rat basophilic leukemia 2H3 (RBL-2H3) cells. Potential allergens were screened by RBL-2H3 cell extraction and allergenicity verified in RBL-2H3 cells, mouse peritoneal mast cells, Laboratory of Allergic Disease 2 (LAD2) human mast cells and mice, respectively. RESULTS: 80% ethanol extract of red ginseng extract induced mast cell degranulation with less cytotoxicity, but 40% ethanol extract could not. Ginsenoside Rd and 20(S)-Rg3 could induce a significant increase in β-hexosaminidase release, histamine release and translocation of phosphatidylserine in RBL-2H3 cells. Ginsenoside Rd and 20(S)-Rg3 also increased β-hexosaminidase release and the intracellular Ca(2+) concentration in mouse peritoneal mast cells and LAD2 cells. In addition, histamine levels in serum of mice were elevated dose-dependently. CONCLUSIONS: Ginsenoside Rd and 20(S)-Rg3 are potential allergens that induce the release of mediators associated with anaphylactoid reactions. Our study could guide optimization of methods associated with Rd/20(S)-Rg3-containing preparations and establishment of quality standards for safe application of Traditional Chinese Medicines. |
format | Online Article Text |
id | pubmed-5490175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54901752017-06-30 Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions Wang, Lu Zhao, Yazheng Yang, Ye Hu, Yuanjia Zou, Xiaohan Yu, Boyang Qi, Jin J Transl Med Research BACKGROUND: Anaphylactoid reactions induced by preparations containing red ginseng have been reported. The aim of this study is to evaluate the allergenicity and screen potential allergens in red ginseng extract thoroughly. METHODS: Red ginseng extract (RGE) and different fractions of RGE were prepared and evaluated by measuring the degranulation and viability of rat basophilic leukemia 2H3 (RBL-2H3) cells. Potential allergens were screened by RBL-2H3 cell extraction and allergenicity verified in RBL-2H3 cells, mouse peritoneal mast cells, Laboratory of Allergic Disease 2 (LAD2) human mast cells and mice, respectively. RESULTS: 80% ethanol extract of red ginseng extract induced mast cell degranulation with less cytotoxicity, but 40% ethanol extract could not. Ginsenoside Rd and 20(S)-Rg3 could induce a significant increase in β-hexosaminidase release, histamine release and translocation of phosphatidylserine in RBL-2H3 cells. Ginsenoside Rd and 20(S)-Rg3 also increased β-hexosaminidase release and the intracellular Ca(2+) concentration in mouse peritoneal mast cells and LAD2 cells. In addition, histamine levels in serum of mice were elevated dose-dependently. CONCLUSIONS: Ginsenoside Rd and 20(S)-Rg3 are potential allergens that induce the release of mediators associated with anaphylactoid reactions. Our study could guide optimization of methods associated with Rd/20(S)-Rg3-containing preparations and establishment of quality standards for safe application of Traditional Chinese Medicines. BioMed Central 2017-06-28 /pmc/articles/PMC5490175/ /pubmed/28659175 http://dx.doi.org/10.1186/s12967-017-1249-x Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Wang, Lu Zhao, Yazheng Yang, Ye Hu, Yuanjia Zou, Xiaohan Yu, Boyang Qi, Jin Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions |
title | Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions |
title_full | Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions |
title_fullStr | Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions |
title_full_unstemmed | Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions |
title_short | Allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions |
title_sort | allergens in red ginseng extract induce the release of mediators associated with anaphylactoid reactions |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490175/ https://www.ncbi.nlm.nih.gov/pubmed/28659175 http://dx.doi.org/10.1186/s12967-017-1249-x |
work_keys_str_mv | AT wanglu allergensinredginsengextractinducethereleaseofmediatorsassociatedwithanaphylactoidreactions AT zhaoyazheng allergensinredginsengextractinducethereleaseofmediatorsassociatedwithanaphylactoidreactions AT yangye allergensinredginsengextractinducethereleaseofmediatorsassociatedwithanaphylactoidreactions AT huyuanjia allergensinredginsengextractinducethereleaseofmediatorsassociatedwithanaphylactoidreactions AT zouxiaohan allergensinredginsengextractinducethereleaseofmediatorsassociatedwithanaphylactoidreactions AT yuboyang allergensinredginsengextractinducethereleaseofmediatorsassociatedwithanaphylactoidreactions AT qijin allergensinredginsengextractinducethereleaseofmediatorsassociatedwithanaphylactoidreactions |