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In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species

BACKGROUND AND PURPOSE: Candida species constitute an important group of opportunistic fungi, which cause various clinical diseases. Considering the resistance of some Candida species to conventional antifungal agents, treatment of such cases may be challenging and complicated. The purpose of this s...

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Autores principales: Mahmoudi, S, Roustaei, M, Zaini, F, Kordbacheh, P, Safara, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Medical Mycology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490307/
https://www.ncbi.nlm.nih.gov/pubmed/28680982
http://dx.doi.org/10.18869/acadpub.cmm.1.2.7
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author Mahmoudi, S
Roustaei, M
Zaini, F
Kordbacheh, P
Safara, M
author_facet Mahmoudi, S
Roustaei, M
Zaini, F
Kordbacheh, P
Safara, M
author_sort Mahmoudi, S
collection PubMed
description BACKGROUND AND PURPOSE: Candida species constitute an important group of opportunistic fungi, which cause various clinical diseases. Considering the resistance of some Candida species to conventional antifungal agents, treatment of such cases may be challenging and complicated. The purpose of this study was to evaluate and compare the antifungal activities of Euphorbia macroclada latex and fluconazole against different Candida species. MATERIALS AND METHODS: A total of 150 Candida isolates including C. albicans (n=77), C. glabrata (n=28), C. parapsilosis (n=23), C. tropicalis (n=15), C. krusei (n=4), C. famata (n=1), C. kefyr (n=1) and C. inconspicua (n=1) were included in this study. In vitro antifungal activities of Euphorbia macroclada latex and fluconazole against these Candida species were evaluated, according to M27-A2 protocol on broth macrodilution method by the Clinical and Laboratory Standards Institute (CLSI). RESULTS: Among 150 Candida isolates, 98 isolates (65.33%), i.e., C. albicans (n=41), C. glabrata (n=23), C. tropicalis (n=12) and C. parapsilosis (n=22) with minimal inhibitory concentration (MIC) ≤ 8 μg/ml were susceptible to fluconazole. Resistance to fluconazole was noted in 15 isolates, i.e., C. albicans (n=10), C. glabrata (n=2), C. krusei (n=1), C. kefyr (n=1), and C. inconspicua (n=1), with MICs of 64 µg/ml. The remaining isolates (n=37) including C. albicans (n=26), C. glabrata (n=3), C. tropicalis (n=3), C. parapsilosis (n=1), C. krusei (n=3) and C. famata (n=1) with MIC= 16-32 µg/ml showed dose-dependent susceptibility. The latex of Euphorbia macroclada was able to inhibit the growth of 30 out of 150 tested Candida isolates with MIC range of 128-512 µg/ml. These isolates were as follows: C. albicans (n=2), C. glabrata (n=4), C. parapsilosis (n=19), C. krusei (n=2) and C. tropicalis (n=3). Compared to other isolates, higher MIC values were noted for C. albicans and C. glabrata (512 µg/ml), respectively. CONCLUSION: The latex of Euphorbia macroclada showed notable antifungal activities against some pathogenic Candida species. Therefore, it can be potentially used as an alternative antifungal agent in future. However, further research is required to identify its active components.
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spelling pubmed-54903072017-07-05 In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species Mahmoudi, S Roustaei, M Zaini, F Kordbacheh, P Safara, M Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Candida species constitute an important group of opportunistic fungi, which cause various clinical diseases. Considering the resistance of some Candida species to conventional antifungal agents, treatment of such cases may be challenging and complicated. The purpose of this study was to evaluate and compare the antifungal activities of Euphorbia macroclada latex and fluconazole against different Candida species. MATERIALS AND METHODS: A total of 150 Candida isolates including C. albicans (n=77), C. glabrata (n=28), C. parapsilosis (n=23), C. tropicalis (n=15), C. krusei (n=4), C. famata (n=1), C. kefyr (n=1) and C. inconspicua (n=1) were included in this study. In vitro antifungal activities of Euphorbia macroclada latex and fluconazole against these Candida species were evaluated, according to M27-A2 protocol on broth macrodilution method by the Clinical and Laboratory Standards Institute (CLSI). RESULTS: Among 150 Candida isolates, 98 isolates (65.33%), i.e., C. albicans (n=41), C. glabrata (n=23), C. tropicalis (n=12) and C. parapsilosis (n=22) with minimal inhibitory concentration (MIC) ≤ 8 μg/ml were susceptible to fluconazole. Resistance to fluconazole was noted in 15 isolates, i.e., C. albicans (n=10), C. glabrata (n=2), C. krusei (n=1), C. kefyr (n=1), and C. inconspicua (n=1), with MICs of 64 µg/ml. The remaining isolates (n=37) including C. albicans (n=26), C. glabrata (n=3), C. tropicalis (n=3), C. parapsilosis (n=1), C. krusei (n=3) and C. famata (n=1) with MIC= 16-32 µg/ml showed dose-dependent susceptibility. The latex of Euphorbia macroclada was able to inhibit the growth of 30 out of 150 tested Candida isolates with MIC range of 128-512 µg/ml. These isolates were as follows: C. albicans (n=2), C. glabrata (n=4), C. parapsilosis (n=19), C. krusei (n=2) and C. tropicalis (n=3). Compared to other isolates, higher MIC values were noted for C. albicans and C. glabrata (512 µg/ml), respectively. CONCLUSION: The latex of Euphorbia macroclada showed notable antifungal activities against some pathogenic Candida species. Therefore, it can be potentially used as an alternative antifungal agent in future. However, further research is required to identify its active components. Iranian Society of Medical Mycology 2015-06 /pmc/articles/PMC5490307/ /pubmed/28680982 http://dx.doi.org/10.18869/acadpub.cmm.1.2.7 Text en Copyright© 2015, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International License ( https://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Original Article
Mahmoudi, S
Roustaei, M
Zaini, F
Kordbacheh, P
Safara, M
In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species
title In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species
title_full In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species
title_fullStr In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species
title_full_unstemmed In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species
title_short In vitro antifungal activities of Euphorbia macroclada and fluconazole against pathogenic Candida species
title_sort in vitro antifungal activities of euphorbia macroclada and fluconazole against pathogenic candida species
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490307/
https://www.ncbi.nlm.nih.gov/pubmed/28680982
http://dx.doi.org/10.18869/acadpub.cmm.1.2.7
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