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The first isolation of Trichosporon coremiiforme from soil in Iran

BACKGROUND AND PURPOSE: Trichosporon is a genus of anamorphic basidiomycetous yeast which is widely distributed in nature and is found in tropical and temperate areas. The aim of this work was to study the isolation, identification and molecular analysis of Trichosporon species in soil. MATERIALS AN...

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Autores principales: Jamali, S, Gharaei, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Medical Mycology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490323/
https://www.ncbi.nlm.nih.gov/pubmed/28680990
http://dx.doi.org/10.18869/acadpub.cmm.1.3.3
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author Jamali, S
Gharaei, M
author_facet Jamali, S
Gharaei, M
author_sort Jamali, S
collection PubMed
description BACKGROUND AND PURPOSE: Trichosporon is a genus of anamorphic basidiomycetous yeast which is widely distributed in nature and is found in tropical and temperate areas. The aim of this work was to study the isolation, identification and molecular analysis of Trichosporon species in soil. MATERIALS AND METHODS: In order to isolate and identify Trichosporon species in soil, 30 samples were collected from 30 different locations across Iran. The isolates were identified by means of the standard methods of yeast identification. To confirm morphological identification, genomic DNA was extracted and the hypervariable D1/D2 domain of the large-subunit (LSU) ribosomal DNA (rDNA) gene was amplified by polymerase chain reaction (PCR), using primer pair NL-1/NL-4, and then the sequences were analyzed. RESULTS: According to the morphological and physiological assessments, isolates were identified as T. coremiiforme. The isolates formed chlamydospore after one week on yeast-malt (YM) agar medium. Using Blast program, we found that the D1/D2 sequences of the T. coremiiforme isolates from Iran (accession no: KP055040 and KP055041) showed 99% homology with the T. coremiiforme deposited in GenBank. All the T. coremiiforme isolates placed in the Ovoides cluster were well-supported by bootstrap values. CONCLUSION: The present study is the first attempt to survey Trichosporon in soil of Iran. To the best of our knowledge, this is the first investigation of T. coremiiforme in Iran.
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spelling pubmed-54903232017-07-05 The first isolation of Trichosporon coremiiforme from soil in Iran Jamali, S Gharaei, M Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Trichosporon is a genus of anamorphic basidiomycetous yeast which is widely distributed in nature and is found in tropical and temperate areas. The aim of this work was to study the isolation, identification and molecular analysis of Trichosporon species in soil. MATERIALS AND METHODS: In order to isolate and identify Trichosporon species in soil, 30 samples were collected from 30 different locations across Iran. The isolates were identified by means of the standard methods of yeast identification. To confirm morphological identification, genomic DNA was extracted and the hypervariable D1/D2 domain of the large-subunit (LSU) ribosomal DNA (rDNA) gene was amplified by polymerase chain reaction (PCR), using primer pair NL-1/NL-4, and then the sequences were analyzed. RESULTS: According to the morphological and physiological assessments, isolates were identified as T. coremiiforme. The isolates formed chlamydospore after one week on yeast-malt (YM) agar medium. Using Blast program, we found that the D1/D2 sequences of the T. coremiiforme isolates from Iran (accession no: KP055040 and KP055041) showed 99% homology with the T. coremiiforme deposited in GenBank. All the T. coremiiforme isolates placed in the Ovoides cluster were well-supported by bootstrap values. CONCLUSION: The present study is the first attempt to survey Trichosporon in soil of Iran. To the best of our knowledge, this is the first investigation of T. coremiiforme in Iran. Iranian Society of Medical Mycology 2015-09 /pmc/articles/PMC5490323/ /pubmed/28680990 http://dx.doi.org/10.18869/acadpub.cmm.1.3.3 Text en Copyright© 2015, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International License ( https://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Original Article
Jamali, S
Gharaei, M
The first isolation of Trichosporon coremiiforme from soil in Iran
title The first isolation of Trichosporon coremiiforme from soil in Iran
title_full The first isolation of Trichosporon coremiiforme from soil in Iran
title_fullStr The first isolation of Trichosporon coremiiforme from soil in Iran
title_full_unstemmed The first isolation of Trichosporon coremiiforme from soil in Iran
title_short The first isolation of Trichosporon coremiiforme from soil in Iran
title_sort first isolation of trichosporon coremiiforme from soil in iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490323/
https://www.ncbi.nlm.nih.gov/pubmed/28680990
http://dx.doi.org/10.18869/acadpub.cmm.1.3.3
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