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Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014
Between July and November 2014, 15 community-acquired cases of Legionnaires´ disease (LD), including four with Legionella pneumophila serogroup 1 sequence type (ST) 82, were diagnosed in Northern Zealand, Denmark. An outbreak was suspected. No ST82 isolates were found in environmental samples and no...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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European Centre for Disease Prevention and Control (ECDC)
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490456/ https://www.ncbi.nlm.nih.gov/pubmed/28662761 http://dx.doi.org/10.2807/1560-7917.ES.2017.22.25.30558 |
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author | Schjørring, Susanne Stegger, Marc Kjelsø, Charlotte Lilje, Berit Bangsborg, Jette M Petersen, Randi F David, Sophia Uldum, Søren A |
author_facet | Schjørring, Susanne Stegger, Marc Kjelsø, Charlotte Lilje, Berit Bangsborg, Jette M Petersen, Randi F David, Sophia Uldum, Søren A |
author_sort | Schjørring, Susanne |
collection | PubMed |
description | Between July and November 2014, 15 community-acquired cases of Legionnaires´ disease (LD), including four with Legionella pneumophila serogroup 1 sequence type (ST) 82, were diagnosed in Northern Zealand, Denmark. An outbreak was suspected. No ST82 isolates were found in environmental samples and no external source was established. Four putative-outbreak ST82 isolates were retrospectively subjected to whole genome sequencing (WGS) followed by phylogenetic analyses with epidemiologically unrelated ST82 sequences. The four putative-outbreak ST82 sequences fell into two clades, the two clades were separated by ca 1,700 single nt polymorphisms (SNP)s when recombination regions were included but only by 12 to 21 SNPs when these were removed. A single putative-outbreak ST82 isolate sequence segregated in the first clade. The other three clustered in the second clade, where all included sequences had < 5 SNP differences between them. Intriguingly, this clade also comprised epidemiologically unrelated isolate sequences from the UK and Denmark dating back as early as 2011. The study confirms that recombination plays a major role in L. pneumophila evolution. On the other hand, strains belonging to the same ST can have only few SNP differences despite being sampled over both large timespans and geographic distances. These are two important factors to consider in outbreak investigations. |
format | Online Article Text |
id | pubmed-5490456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | European Centre for Disease Prevention and Control (ECDC) |
record_format | MEDLINE/PubMed |
spelling | pubmed-54904562017-07-11 Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014 Schjørring, Susanne Stegger, Marc Kjelsø, Charlotte Lilje, Berit Bangsborg, Jette M Petersen, Randi F David, Sophia Uldum, Søren A Euro Surveill Surveillance and Outbreak Report Between July and November 2014, 15 community-acquired cases of Legionnaires´ disease (LD), including four with Legionella pneumophila serogroup 1 sequence type (ST) 82, were diagnosed in Northern Zealand, Denmark. An outbreak was suspected. No ST82 isolates were found in environmental samples and no external source was established. Four putative-outbreak ST82 isolates were retrospectively subjected to whole genome sequencing (WGS) followed by phylogenetic analyses with epidemiologically unrelated ST82 sequences. The four putative-outbreak ST82 sequences fell into two clades, the two clades were separated by ca 1,700 single nt polymorphisms (SNP)s when recombination regions were included but only by 12 to 21 SNPs when these were removed. A single putative-outbreak ST82 isolate sequence segregated in the first clade. The other three clustered in the second clade, where all included sequences had < 5 SNP differences between them. Intriguingly, this clade also comprised epidemiologically unrelated isolate sequences from the UK and Denmark dating back as early as 2011. The study confirms that recombination plays a major role in L. pneumophila evolution. On the other hand, strains belonging to the same ST can have only few SNP differences despite being sampled over both large timespans and geographic distances. These are two important factors to consider in outbreak investigations. European Centre for Disease Prevention and Control (ECDC) 2017-06-22 /pmc/articles/PMC5490456/ /pubmed/28662761 http://dx.doi.org/10.2807/1560-7917.ES.2017.22.25.30558 Text en This article is copyright of The Authors, 2017. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made. |
spellingShingle | Surveillance and Outbreak Report Schjørring, Susanne Stegger, Marc Kjelsø, Charlotte Lilje, Berit Bangsborg, Jette M Petersen, Randi F David, Sophia Uldum, Søren A Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014 |
title | Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014 |
title_full | Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014 |
title_fullStr | Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014 |
title_full_unstemmed | Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014 |
title_short | Genomic investigation of a suspected outbreak of Legionella pneumophila ST82 reveals undetected heterogeneity by the present gold-standard methods, Denmark, July to November 2014 |
title_sort | genomic investigation of a suspected outbreak of legionella pneumophila st82 reveals undetected heterogeneity by the present gold-standard methods, denmark, july to november 2014 |
topic | Surveillance and Outbreak Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490456/ https://www.ncbi.nlm.nih.gov/pubmed/28662761 http://dx.doi.org/10.2807/1560-7917.ES.2017.22.25.30558 |
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