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(−)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway

The beneficial effects of tea consumption on cancer prevention have been generally reported, while (−)-Epigallocatechin-3-gallate (EGCG) is the major active component from green tea. Cancer stem cells (CSCs) play a crucial role in the process of cancer development. Targeting CSCs may be an effective...

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Autores principales: Chen, Yue, Wang, Xiao-Qian, Zhang, Qi, Zhu, Jian-Yun, Li, Yuan, Xie, Chun-Feng, Li, Xiao-Ting, Wu, Jie-Shu, Geng, Shan-Shan, Zhong, Cai-Yun, Han, Hong-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490551/
https://www.ncbi.nlm.nih.gov/pubmed/28587207
http://dx.doi.org/10.3390/nu9060572
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author Chen, Yue
Wang, Xiao-Qian
Zhang, Qi
Zhu, Jian-Yun
Li, Yuan
Xie, Chun-Feng
Li, Xiao-Ting
Wu, Jie-Shu
Geng, Shan-Shan
Zhong, Cai-Yun
Han, Hong-Yu
author_facet Chen, Yue
Wang, Xiao-Qian
Zhang, Qi
Zhu, Jian-Yun
Li, Yuan
Xie, Chun-Feng
Li, Xiao-Ting
Wu, Jie-Shu
Geng, Shan-Shan
Zhong, Cai-Yun
Han, Hong-Yu
author_sort Chen, Yue
collection PubMed
description The beneficial effects of tea consumption on cancer prevention have been generally reported, while (−)-Epigallocatechin-3-gallate (EGCG) is the major active component from green tea. Cancer stem cells (CSCs) play a crucial role in the process of cancer development. Targeting CSCs may be an effective way for cancer intervention. However, the effects of EGCG on colorectal CSCs and the underlying mechanisms remain unclear. Spheroid formation assay was used to enrich colorectal CSCs from colorectal cancer cell lines. Immunoblotting analysis and quantitative real-time polymerase chain reaction were used to measure the alterations of critical molecules expression. Immunofluorescence staining analysis was also used to determine the expression of CD133. We revealed that EGCG inhibited the spheroid formation capability of colorectal cancer cells as well as the expression of colorectal CSC markers, along with suppression of cell proliferation and induction of apoptosis. Moreover, we illustrated that EGCG downregulated the activation of Wnt/β-catenin pathway, while upregulation of Wnt/β-catenin diminished the inhibitory effects of EGCG on colorectal CSCs. Taken together, this study suggested that EGCG could be an effective natural compound targeting colorectal CSCs through suppression of Wnt/β-catenin pathway, and thus may be a promising agent for colorectal cancer intervention.
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spelling pubmed-54905512017-07-03 (−)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway Chen, Yue Wang, Xiao-Qian Zhang, Qi Zhu, Jian-Yun Li, Yuan Xie, Chun-Feng Li, Xiao-Ting Wu, Jie-Shu Geng, Shan-Shan Zhong, Cai-Yun Han, Hong-Yu Nutrients Article The beneficial effects of tea consumption on cancer prevention have been generally reported, while (−)-Epigallocatechin-3-gallate (EGCG) is the major active component from green tea. Cancer stem cells (CSCs) play a crucial role in the process of cancer development. Targeting CSCs may be an effective way for cancer intervention. However, the effects of EGCG on colorectal CSCs and the underlying mechanisms remain unclear. Spheroid formation assay was used to enrich colorectal CSCs from colorectal cancer cell lines. Immunoblotting analysis and quantitative real-time polymerase chain reaction were used to measure the alterations of critical molecules expression. Immunofluorescence staining analysis was also used to determine the expression of CD133. We revealed that EGCG inhibited the spheroid formation capability of colorectal cancer cells as well as the expression of colorectal CSC markers, along with suppression of cell proliferation and induction of apoptosis. Moreover, we illustrated that EGCG downregulated the activation of Wnt/β-catenin pathway, while upregulation of Wnt/β-catenin diminished the inhibitory effects of EGCG on colorectal CSCs. Taken together, this study suggested that EGCG could be an effective natural compound targeting colorectal CSCs through suppression of Wnt/β-catenin pathway, and thus may be a promising agent for colorectal cancer intervention. MDPI 2017-06-03 /pmc/articles/PMC5490551/ /pubmed/28587207 http://dx.doi.org/10.3390/nu9060572 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Yue
Wang, Xiao-Qian
Zhang, Qi
Zhu, Jian-Yun
Li, Yuan
Xie, Chun-Feng
Li, Xiao-Ting
Wu, Jie-Shu
Geng, Shan-Shan
Zhong, Cai-Yun
Han, Hong-Yu
(−)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway
title (−)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway
title_full (−)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway
title_fullStr (−)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway
title_full_unstemmed (−)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway
title_short (−)-Epigallocatechin-3-Gallate Inhibits Colorectal Cancer Stem Cells by Suppressing Wnt/β-Catenin Pathway
title_sort (−)-epigallocatechin-3-gallate inhibits colorectal cancer stem cells by suppressing wnt/β-catenin pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490551/
https://www.ncbi.nlm.nih.gov/pubmed/28587207
http://dx.doi.org/10.3390/nu9060572
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