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Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis

Guarana (Paullinia cupana) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guaran...

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Autores principales: Lima, Natália da Silva, Numata, Erica de Paula, Mesquita, Leonardo Mendes de Souza, Dias, Pollyana Hammoud, Vilegas, Wagner, Gambero, Alessandra, Ribeiro, Marcelo Lima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490614/
https://www.ncbi.nlm.nih.gov/pubmed/28632199
http://dx.doi.org/10.3390/nu9060635
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author Lima, Natália da Silva
Numata, Erica de Paula
Mesquita, Leonardo Mendes de Souza
Dias, Pollyana Hammoud
Vilegas, Wagner
Gambero, Alessandra
Ribeiro, Marcelo Lima
author_facet Lima, Natália da Silva
Numata, Erica de Paula
Mesquita, Leonardo Mendes de Souza
Dias, Pollyana Hammoud
Vilegas, Wagner
Gambero, Alessandra
Ribeiro, Marcelo Lima
author_sort Lima, Natália da Silva
collection PubMed
description Guarana (Paullinia cupana) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (−12%, −20%, −24% and −40%, respectively, p < 0.0001). An up-regulation of the anti-adipogenic genes Wnt10b, Wnt3a, Wnt1, Gata3 and Dlk1 and a down-regulation of pro-adipogenic genes Cebpα, Pparγ and Creb1 were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets Wnt3a, Wnt1 and Wnt10b. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (p < 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent.
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spelling pubmed-54906142017-07-03 Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis Lima, Natália da Silva Numata, Erica de Paula Mesquita, Leonardo Mendes de Souza Dias, Pollyana Hammoud Vilegas, Wagner Gambero, Alessandra Ribeiro, Marcelo Lima Nutrients Article Guarana (Paullinia cupana) is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (−12%, −20%, −24% and −40%, respectively, p < 0.0001). An up-regulation of the anti-adipogenic genes Wnt10b, Wnt3a, Wnt1, Gata3 and Dlk1 and a down-regulation of pro-adipogenic genes Cebpα, Pparγ and Creb1 were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets Wnt3a, Wnt1 and Wnt10b. Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation (p < 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent. MDPI 2017-06-20 /pmc/articles/PMC5490614/ /pubmed/28632199 http://dx.doi.org/10.3390/nu9060635 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lima, Natália da Silva
Numata, Erica de Paula
Mesquita, Leonardo Mendes de Souza
Dias, Pollyana Hammoud
Vilegas, Wagner
Gambero, Alessandra
Ribeiro, Marcelo Lima
Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis
title Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis
title_full Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis
title_fullStr Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis
title_full_unstemmed Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis
title_short Modulatory Effects of Guarana (Paullinia cupana) on Adipogenesis
title_sort modulatory effects of guarana (paullinia cupana) on adipogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490614/
https://www.ncbi.nlm.nih.gov/pubmed/28632199
http://dx.doi.org/10.3390/nu9060635
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