Microarray-based resequencing of multiple Bacillus anthracis isolates

We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 × 10(-7)) and by comparison to independently generated shotgun sequence (discrepancy ra...

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Detalles Bibliográficos
Autores principales: Zwick, Michael E, Mcafee, Farrell, Cutler, David J, Read, Timothy D, Ravel, Jacques, Bowman, Gregory R, Galloway, Darrell R, Mateczun, Alfred
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549062/
https://www.ncbi.nlm.nih.gov/pubmed/15642093
http://dx.doi.org/10.1186/gb-2004-6-1-r10
Descripción
Sumario:We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 × 10(-7)) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 × 10(-6)). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains.

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