Microarray-based resequencing of multiple Bacillus anthracis isolates

We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 × 10(-7)) and by comparison to independently generated shotgun sequence (discrepancy ra...

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Detalles Bibliográficos
Autores principales: Zwick, Michael E, Mcafee, Farrell, Cutler, David J, Read, Timothy D, Ravel, Jacques, Bowman, Gregory R, Galloway, Darrell R, Mateczun, Alfred
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549062/
https://www.ncbi.nlm.nih.gov/pubmed/15642093
http://dx.doi.org/10.1186/gb-2004-6-1-r10
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author Zwick, Michael E
Mcafee, Farrell
Cutler, David J
Read, Timothy D
Ravel, Jacques
Bowman, Gregory R
Galloway, Darrell R
Mateczun, Alfred
author_facet Zwick, Michael E
Mcafee, Farrell
Cutler, David J
Read, Timothy D
Ravel, Jacques
Bowman, Gregory R
Galloway, Darrell R
Mateczun, Alfred
author_sort Zwick, Michael E
collection PubMed
description We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 × 10(-7)) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 × 10(-6)). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains.
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spelling pubmed-5490622005-02-18 Microarray-based resequencing of multiple Bacillus anthracis isolates Zwick, Michael E Mcafee, Farrell Cutler, David J Read, Timothy D Ravel, Jacques Bowman, Gregory R Galloway, Darrell R Mateczun, Alfred Genome Biol Method We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 × 10(-7)) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 × 10(-6)). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains. BioMed Central 2005 2004-12-17 /pmc/articles/PMC549062/ /pubmed/15642093 http://dx.doi.org/10.1186/gb-2004-6-1-r10 Text en Copyright © 2004 Zwick et al.; licensee BioMed Central Ltd.
spellingShingle Method
Zwick, Michael E
Mcafee, Farrell
Cutler, David J
Read, Timothy D
Ravel, Jacques
Bowman, Gregory R
Galloway, Darrell R
Mateczun, Alfred
Microarray-based resequencing of multiple Bacillus anthracis isolates
title Microarray-based resequencing of multiple Bacillus anthracis isolates
title_full Microarray-based resequencing of multiple Bacillus anthracis isolates
title_fullStr Microarray-based resequencing of multiple Bacillus anthracis isolates
title_full_unstemmed Microarray-based resequencing of multiple Bacillus anthracis isolates
title_short Microarray-based resequencing of multiple Bacillus anthracis isolates
title_sort microarray-based resequencing of multiple bacillus anthracis isolates
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549062/
https://www.ncbi.nlm.nih.gov/pubmed/15642093
http://dx.doi.org/10.1186/gb-2004-6-1-r10
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