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Wound healing and inflammation genes revealed by array analysis of 'macrophageless' PU.1 null mice

BACKGROUND: Wound healing is a complex process requiring the collaborative efforts of different tissues and cell lineages, and involving the coordinated interplay of several phases of proliferation, migration, matrix synthesis and contraction. Tissue damage also triggers a robust influx of inflammat...

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Autores principales: Cooper, Lisa, Johnson, Claire, Burslem, Frank, Martin, Paul
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549066/
https://www.ncbi.nlm.nih.gov/pubmed/15642097
http://dx.doi.org/10.1186/gb-2004-6-1-r5
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author Cooper, Lisa
Johnson, Claire
Burslem, Frank
Martin, Paul
author_facet Cooper, Lisa
Johnson, Claire
Burslem, Frank
Martin, Paul
author_sort Cooper, Lisa
collection PubMed
description BACKGROUND: Wound healing is a complex process requiring the collaborative efforts of different tissues and cell lineages, and involving the coordinated interplay of several phases of proliferation, migration, matrix synthesis and contraction. Tissue damage also triggers a robust influx of inflammatory leukocytes to the wound site that play key roles in clearing the wound of invading microbes but also release signals that may be detrimental to repair and lead to fibrosis. RESULTS: To better define key cellular events pivotal for tissue repair yet independent of inflammation we have used a microarray approach to determine a portfolio of over 1,000 genes expressed across the repair response in a wild-type neonatal mouse versus its PU.1 null sib. The PU.1 null mouse is genetically incapable of raising the standard inflammatory response, because it lacks macrophages and functioning neutrophils, yet repairs skin wounds rapidly and with reduced fibrosis. Conversely, by subtraction, we have determined genes that are either expressed by leukocytes, or upregulated by fibroblasts, endothelial cells, muscle cells and others at the wound site, as a consequence of inflammation. To determine the spatial expression pattern for several genes in each cluster we have also performed in situ hybridization studies. CONCLUSIONS: Cluster analysis of genes expressed after wounding wild-type mice versus PU.1 null sibs distinguishes between tissue repair genes and genes associated with inflammation and its consequences. Our data reveal and classify several pools of genes, giving insight into their likely functions during repair and hinting at potential therapeutic targets.
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spelling pubmed-5490662005-02-18 Wound healing and inflammation genes revealed by array analysis of 'macrophageless' PU.1 null mice Cooper, Lisa Johnson, Claire Burslem, Frank Martin, Paul Genome Biol Research BACKGROUND: Wound healing is a complex process requiring the collaborative efforts of different tissues and cell lineages, and involving the coordinated interplay of several phases of proliferation, migration, matrix synthesis and contraction. Tissue damage also triggers a robust influx of inflammatory leukocytes to the wound site that play key roles in clearing the wound of invading microbes but also release signals that may be detrimental to repair and lead to fibrosis. RESULTS: To better define key cellular events pivotal for tissue repair yet independent of inflammation we have used a microarray approach to determine a portfolio of over 1,000 genes expressed across the repair response in a wild-type neonatal mouse versus its PU.1 null sib. The PU.1 null mouse is genetically incapable of raising the standard inflammatory response, because it lacks macrophages and functioning neutrophils, yet repairs skin wounds rapidly and with reduced fibrosis. Conversely, by subtraction, we have determined genes that are either expressed by leukocytes, or upregulated by fibroblasts, endothelial cells, muscle cells and others at the wound site, as a consequence of inflammation. To determine the spatial expression pattern for several genes in each cluster we have also performed in situ hybridization studies. CONCLUSIONS: Cluster analysis of genes expressed after wounding wild-type mice versus PU.1 null sibs distinguishes between tissue repair genes and genes associated with inflammation and its consequences. Our data reveal and classify several pools of genes, giving insight into their likely functions during repair and hinting at potential therapeutic targets. BioMed Central 2005 2004-12-23 /pmc/articles/PMC549066/ /pubmed/15642097 http://dx.doi.org/10.1186/gb-2004-6-1-r5 Text en Copyright © 2004 Cooper et al.; licensee BioMed Central Ltd.
spellingShingle Research
Cooper, Lisa
Johnson, Claire
Burslem, Frank
Martin, Paul
Wound healing and inflammation genes revealed by array analysis of 'macrophageless' PU.1 null mice
title Wound healing and inflammation genes revealed by array analysis of 'macrophageless' PU.1 null mice
title_full Wound healing and inflammation genes revealed by array analysis of 'macrophageless' PU.1 null mice
title_fullStr Wound healing and inflammation genes revealed by array analysis of 'macrophageless' PU.1 null mice
title_full_unstemmed Wound healing and inflammation genes revealed by array analysis of 'macrophageless' PU.1 null mice
title_short Wound healing and inflammation genes revealed by array analysis of 'macrophageless' PU.1 null mice
title_sort wound healing and inflammation genes revealed by array analysis of 'macrophageless' pu.1 null mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549066/
https://www.ncbi.nlm.nih.gov/pubmed/15642097
http://dx.doi.org/10.1186/gb-2004-6-1-r5
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