Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these ge...

Descripción completa

Detalles Bibliográficos
Autores principales: Radonić, Aleksandar, Thulke, Stefanie, Bae, Hi-Gung, Müller, Marcel A, Siegert, Wolfgang, Nitsche, Andreas
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549079/
https://www.ncbi.nlm.nih.gov/pubmed/15705200
http://dx.doi.org/10.1186/1743-422X-2-7
_version_ 1782122390639083520
author Radonić, Aleksandar
Thulke, Stefanie
Bae, Hi-Gung
Müller, Marcel A
Siegert, Wolfgang
Nitsche, Andreas
author_facet Radonić, Aleksandar
Thulke, Stefanie
Bae, Hi-Gung
Müller, Marcel A
Siegert, Wolfgang
Nitsche, Andreas
author_sort Radonić, Aleksandar
collection PubMed
description Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.
format Text
id pubmed-549079
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-5490792005-02-19 Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Radonić, Aleksandar Thulke, Stefanie Bae, Hi-Gung Müller, Marcel A Siegert, Wolfgang Nitsche, Andreas Virol J Research Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells. BioMed Central 2005-02-10 /pmc/articles/PMC549079/ /pubmed/15705200 http://dx.doi.org/10.1186/1743-422X-2-7 Text en Copyright © 2005 Radonić et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Radonić, Aleksandar
Thulke, Stefanie
Bae, Hi-Gung
Müller, Marcel A
Siegert, Wolfgang
Nitsche, Andreas
Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
title Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
title_full Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
title_fullStr Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
title_full_unstemmed Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
title_short Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
title_sort reference gene selection for quantitative real-time pcr analysis in virus infected cells: sars corona virus, yellow fever virus, human herpesvirus-6, camelpox virus and cytomegalovirus infections
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549079/
https://www.ncbi.nlm.nih.gov/pubmed/15705200
http://dx.doi.org/10.1186/1743-422X-2-7
work_keys_str_mv AT radonicaleksandar referencegeneselectionforquantitativerealtimepcranalysisinvirusinfectedcellssarscoronavirusyellowfevervirushumanherpesvirus6camelpoxvirusandcytomegalovirusinfections
AT thulkestefanie referencegeneselectionforquantitativerealtimepcranalysisinvirusinfectedcellssarscoronavirusyellowfevervirushumanherpesvirus6camelpoxvirusandcytomegalovirusinfections
AT baehigung referencegeneselectionforquantitativerealtimepcranalysisinvirusinfectedcellssarscoronavirusyellowfevervirushumanherpesvirus6camelpoxvirusandcytomegalovirusinfections
AT mullermarcela referencegeneselectionforquantitativerealtimepcranalysisinvirusinfectedcellssarscoronavirusyellowfevervirushumanherpesvirus6camelpoxvirusandcytomegalovirusinfections
AT siegertwolfgang referencegeneselectionforquantitativerealtimepcranalysisinvirusinfectedcellssarscoronavirusyellowfevervirushumanherpesvirus6camelpoxvirusandcytomegalovirusinfections
AT nitscheandreas referencegeneselectionforquantitativerealtimepcranalysisinvirusinfectedcellssarscoronavirusyellowfevervirushumanherpesvirus6camelpoxvirusandcytomegalovirusinfections

Ejemplares similares