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Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans
Context: The twigs of Sorbus alnifolia (Sieb. et Zucc.) K. Koch (Rosaceae) have been used to treat neurological disorders as a traditional medicine in Korea. However, there are limited data describing the efficacy of S. alnifolia in Parkinson’s disease (PD). Objective: This study was conducted to id...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490792/ https://www.ncbi.nlm.nih.gov/pubmed/27937005 http://dx.doi.org/10.1080/13880209.2016.1251468 |
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author | Cheon, Se-Myeong Jang, Insoo Lee, Myon-Hee Kim, Dae Keun Jeon, Hoon Cha, Dong Seok |
author_facet | Cheon, Se-Myeong Jang, Insoo Lee, Myon-Hee Kim, Dae Keun Jeon, Hoon Cha, Dong Seok |
author_sort | Cheon, Se-Myeong |
collection | PubMed |
description | Context: The twigs of Sorbus alnifolia (Sieb. et Zucc.) K. Koch (Rosaceae) have been used to treat neurological disorders as a traditional medicine in Korea. However, there are limited data describing the efficacy of S. alnifolia in Parkinson’s disease (PD). Objective: This study was conducted to identify the protective effects of the methanol extracts of S. alnifolia (MESA) on the dopaminergic (DA) neurodegeneration in Caenorhabditis elegans. Materials and methods: To test the neuroprotective action of MESA, viability assay was performed after 48 h exposure to 1-methyl-4-phenylpyridine (MMP(+)) in PC12 cells and C. elegans (400 μM and 2 mM of MMP(+), respectively). Fluorescence intensity was quantified using transgenic mutants such as BZ555 (Pdat-1::GFP) and and UA57 (Pdat-1::GFP and Pdat-1::CAT-2) to determine MESA’s effects on DA neurodegeneration in C. elegans. Aggregation of α-synuclein was observed using NL5901 strain (unc-54p::α-synuclein::YFP). MESA’s protective effects on the DA neuronal functions were examined by food-sensing assay. Lifespan assay was conducted to test the effects of MESA on the longevity. Results: MESA restored MPP(+)-induced loss of viability in both PC12 cells and C. elegans (85.8% and 54.9%, respectively). In C. elegans, MESA provided protection against chemically and genetically-induced DA neurodegeneration, respectively. Moreover, food-sensing functions were increased 58.4% by MESA in the DA neuron degraded worms. MESA also prolonged the average lifespan by 25.6%. However, MESA failed to alter α-synuclein aggregation. Discussion and conclusions: These results revealed that MESA protects DA neurodegeneration and recovers diminished DA neuronal functions, thereby can be a valuable candidate for the treatment of PD. |
format | Online Article Text |
id | pubmed-5490792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-54907922017-12-01 Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans Cheon, Se-Myeong Jang, Insoo Lee, Myon-Hee Kim, Dae Keun Jeon, Hoon Cha, Dong Seok Pharm Biol Research Article Context: The twigs of Sorbus alnifolia (Sieb. et Zucc.) K. Koch (Rosaceae) have been used to treat neurological disorders as a traditional medicine in Korea. However, there are limited data describing the efficacy of S. alnifolia in Parkinson’s disease (PD). Objective: This study was conducted to identify the protective effects of the methanol extracts of S. alnifolia (MESA) on the dopaminergic (DA) neurodegeneration in Caenorhabditis elegans. Materials and methods: To test the neuroprotective action of MESA, viability assay was performed after 48 h exposure to 1-methyl-4-phenylpyridine (MMP(+)) in PC12 cells and C. elegans (400 μM and 2 mM of MMP(+), respectively). Fluorescence intensity was quantified using transgenic mutants such as BZ555 (Pdat-1::GFP) and and UA57 (Pdat-1::GFP and Pdat-1::CAT-2) to determine MESA’s effects on DA neurodegeneration in C. elegans. Aggregation of α-synuclein was observed using NL5901 strain (unc-54p::α-synuclein::YFP). MESA’s protective effects on the DA neuronal functions were examined by food-sensing assay. Lifespan assay was conducted to test the effects of MESA on the longevity. Results: MESA restored MPP(+)-induced loss of viability in both PC12 cells and C. elegans (85.8% and 54.9%, respectively). In C. elegans, MESA provided protection against chemically and genetically-induced DA neurodegeneration, respectively. Moreover, food-sensing functions were increased 58.4% by MESA in the DA neuron degraded worms. MESA also prolonged the average lifespan by 25.6%. However, MESA failed to alter α-synuclein aggregation. Discussion and conclusions: These results revealed that MESA protects DA neurodegeneration and recovers diminished DA neuronal functions, thereby can be a valuable candidate for the treatment of PD. Taylor & Francis 2016-12-09 /pmc/articles/PMC5490792/ /pubmed/27937005 http://dx.doi.org/10.1080/13880209.2016.1251468 Text en © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cheon, Se-Myeong Jang, Insoo Lee, Myon-Hee Kim, Dae Keun Jeon, Hoon Cha, Dong Seok Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans |
title | Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans |
title_full | Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans |
title_fullStr | Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans |
title_full_unstemmed | Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans |
title_short | Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans |
title_sort | sorbus alnifolia protects dopaminergic neurodegeneration in caenorhabditis elegans |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5490792/ https://www.ncbi.nlm.nih.gov/pubmed/27937005 http://dx.doi.org/10.1080/13880209.2016.1251468 |
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