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Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry
The classification of melanoma into four histological subtypes has been questioned regarding its clinical validity in providing relevant information for treatment for metastatic tumors. Specific genetic alterations are associated with particular clinical and histopathological features, suggesting th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491027/ https://www.ncbi.nlm.nih.gov/pubmed/28662062 http://dx.doi.org/10.1371/journal.pone.0179745 |
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author | Urvanegia, Ana Claudia Tavoloni Braga, Juliana Casagrande Shitara, Danielle Fregnani, Jose Humberto Neves, Jose Ivanildo Pinto, Clovis Antonio Marghoob, Ashfaq A. Duprat, Joao Pedreira Rezze, Gisele Gargantini |
author_facet | Urvanegia, Ana Claudia Tavoloni Braga, Juliana Casagrande Shitara, Danielle Fregnani, Jose Humberto Neves, Jose Ivanildo Pinto, Clovis Antonio Marghoob, Ashfaq A. Duprat, Joao Pedreira Rezze, Gisele Gargantini |
author_sort | Urvanegia, Ana Claudia |
collection | PubMed |
description | The classification of melanoma into four histological subtypes has been questioned regarding its clinical validity in providing relevant information for treatment for metastatic tumors. Specific genetic alterations are associated with particular clinical and histopathological features, suggesting that these could be helpful in refining existing melanoma classification schemes. We analyzed BRAF V600E mutated melanomas to explore the Reflectance confocal microscopy (RCM) utility as a screening aid in the evaluation of the most appropriate patients for genetic testing. Thus, 32 melanomas were assessed regarding their BRAF V600E mutational status. Experts blinded to dermoscopic images and V600E immunohistochemistry results evaluated RCM images regarding previously described melanoma features. BRAF positive melanomas were related to younger age (p = 0.035), invasive melanomas (p = 0.03) and to the presence of hiporreflective cells (p = 0.02), epidermal nests (p = 0.02), dermal-epidermal junction nests (p = 0.05), edged papillae (p = 0.05), and bright dots (p = 0.05), and to absence of junctional thickening due to isolated cells (p = 0.01) and meshwork (p = 0.02). This study can not characterize other mutations in the BRAF, because the immunohistochemistry is specific to the type V600E. The findings should encourage the genetic evaluation of BRAF mutation. This study highlights the potential of RCM as a supplementary tool in the screening of BRAF-mutated melanomas. |
format | Online Article Text |
id | pubmed-5491027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54910272017-07-18 Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry Urvanegia, Ana Claudia Tavoloni Braga, Juliana Casagrande Shitara, Danielle Fregnani, Jose Humberto Neves, Jose Ivanildo Pinto, Clovis Antonio Marghoob, Ashfaq A. Duprat, Joao Pedreira Rezze, Gisele Gargantini PLoS One Research Article The classification of melanoma into four histological subtypes has been questioned regarding its clinical validity in providing relevant information for treatment for metastatic tumors. Specific genetic alterations are associated with particular clinical and histopathological features, suggesting that these could be helpful in refining existing melanoma classification schemes. We analyzed BRAF V600E mutated melanomas to explore the Reflectance confocal microscopy (RCM) utility as a screening aid in the evaluation of the most appropriate patients for genetic testing. Thus, 32 melanomas were assessed regarding their BRAF V600E mutational status. Experts blinded to dermoscopic images and V600E immunohistochemistry results evaluated RCM images regarding previously described melanoma features. BRAF positive melanomas were related to younger age (p = 0.035), invasive melanomas (p = 0.03) and to the presence of hiporreflective cells (p = 0.02), epidermal nests (p = 0.02), dermal-epidermal junction nests (p = 0.05), edged papillae (p = 0.05), and bright dots (p = 0.05), and to absence of junctional thickening due to isolated cells (p = 0.01) and meshwork (p = 0.02). This study can not characterize other mutations in the BRAF, because the immunohistochemistry is specific to the type V600E. The findings should encourage the genetic evaluation of BRAF mutation. This study highlights the potential of RCM as a supplementary tool in the screening of BRAF-mutated melanomas. Public Library of Science 2017-06-29 /pmc/articles/PMC5491027/ /pubmed/28662062 http://dx.doi.org/10.1371/journal.pone.0179745 Text en © 2017 Urvanegia et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Urvanegia, Ana Claudia Tavoloni Braga, Juliana Casagrande Shitara, Danielle Fregnani, Jose Humberto Neves, Jose Ivanildo Pinto, Clovis Antonio Marghoob, Ashfaq A. Duprat, Joao Pedreira Rezze, Gisele Gargantini Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry |
title | Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry |
title_full | Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry |
title_fullStr | Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry |
title_full_unstemmed | Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry |
title_short | Reflectance confocal microscopy features of BRAF V600E mutated thin melanomas detected by immunohistochemistry |
title_sort | reflectance confocal microscopy features of braf v600e mutated thin melanomas detected by immunohistochemistry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491027/ https://www.ncbi.nlm.nih.gov/pubmed/28662062 http://dx.doi.org/10.1371/journal.pone.0179745 |
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