Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design

Establishing stable Chinese Hamster Ovary (CHO) cells producing monoclonal antibodies (mAbs) usually pass through the random integration of vectors to the cell genome, which is sensitive to gene silencing. One approach to overcome this issue is to target a highly transcribed region in the genome. Tr...

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Detalles Bibliográficos
Autores principales: Ahmadi, Samira, Davami, Fatemeh, Davoudi, Noushin, Nematpour, Fatemeh, Ahmadi, Maryam, Ebadat, Saeedeh, Azadmanesh, Kayhan, Barkhordari, Farzaneh, Mahboudi, Fereidoun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491063/
https://www.ncbi.nlm.nih.gov/pubmed/28662065
http://dx.doi.org/10.1371/journal.pone.0179902

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491063/
https://www.ncbi.nlm.nih.gov/pubmed/28662065
http://dx.doi.org/10.1371/journal.pone.0179902

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