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Circulating tumor cells detected by lab-on-a-disc: Role in early diagnosis of gastric cancer

BACKGROUND: The use of circulating tumor cells (CTCs) as an early diagnostic biomarker and prognostic indicator after surgery or chemotherapy has been suggested for various cancers. This study aimed to evaluate CTCs in patients who underwent gastrectomy for gastric cancer and to explore their clinic...

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Autores principales: Kang, Hwa Mi, Kim, Gwang Ha, Jeon, Hye Kyung, Kim, Dae Hwan, Jeon, Tae Yong, Park, Do Youn, Jeong, Hyunjin, Chun, Won Joo, Kim, Mi-Hyun, Park, Juhee, Lim, Minji, Kim, Tae-Hyeong, Cho, Yoon-Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491173/
https://www.ncbi.nlm.nih.gov/pubmed/28662130
http://dx.doi.org/10.1371/journal.pone.0180251
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author Kang, Hwa Mi
Kim, Gwang Ha
Jeon, Hye Kyung
Kim, Dae Hwan
Jeon, Tae Yong
Park, Do Youn
Jeong, Hyunjin
Chun, Won Joo
Kim, Mi-Hyun
Park, Juhee
Lim, Minji
Kim, Tae-Hyeong
Cho, Yoon-Kyung
author_facet Kang, Hwa Mi
Kim, Gwang Ha
Jeon, Hye Kyung
Kim, Dae Hwan
Jeon, Tae Yong
Park, Do Youn
Jeong, Hyunjin
Chun, Won Joo
Kim, Mi-Hyun
Park, Juhee
Lim, Minji
Kim, Tae-Hyeong
Cho, Yoon-Kyung
author_sort Kang, Hwa Mi
collection PubMed
description BACKGROUND: The use of circulating tumor cells (CTCs) as an early diagnostic biomarker and prognostic indicator after surgery or chemotherapy has been suggested for various cancers. This study aimed to evaluate CTCs in patients who underwent gastrectomy for gastric cancer and to explore their clinical usefulness in the early diagnosis of gastric cancer. METHODS: A total of 116 patients with gastric cancer who underwent gastrectomy and 31 healthy volunteers were prospectively included between 2014 and 2015. Peripheral blood samples were collected before gastrectomy, and CTCs were examined using a centrifugal microfluidic system with a new fluid-assisted separation technique. RESULTS: After creating a receiver operating characteristic curve to identify the discriminative CTC value needed differentiate patients with gastric cancer from healthy volunteers, sensitivity and specificity were nearly optimized at a CTC threshold of 2 per 7.5 mL of blood. Of the 102 persons with a CTC level ≥2 per 7.5 mL of blood, 99 (97.1%) had gastric cancer, and of the 45 persons with a CTC level <2 per 7.5 mL of blood, 28 (62.2%) were healthy controls. Accordingly, the sensitivity and specificity for the differentiation of patients with gastric cancer from healthy controls were 85.3% and 90.3%, respectively. However, the presence of CTCs was not associated with any clinicopathologic features such as staging, histologic type, or mucin phenotype. CONCLUSION: Although we could not prove the clinical feasibility of CTCs for gastric cancer staging, our results suggest a potential role of CTCs as an early diagnostic biomarker of gastric cancer.
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spelling pubmed-54911732017-07-18 Circulating tumor cells detected by lab-on-a-disc: Role in early diagnosis of gastric cancer Kang, Hwa Mi Kim, Gwang Ha Jeon, Hye Kyung Kim, Dae Hwan Jeon, Tae Yong Park, Do Youn Jeong, Hyunjin Chun, Won Joo Kim, Mi-Hyun Park, Juhee Lim, Minji Kim, Tae-Hyeong Cho, Yoon-Kyung PLoS One Research Article BACKGROUND: The use of circulating tumor cells (CTCs) as an early diagnostic biomarker and prognostic indicator after surgery or chemotherapy has been suggested for various cancers. This study aimed to evaluate CTCs in patients who underwent gastrectomy for gastric cancer and to explore their clinical usefulness in the early diagnosis of gastric cancer. METHODS: A total of 116 patients with gastric cancer who underwent gastrectomy and 31 healthy volunteers were prospectively included between 2014 and 2015. Peripheral blood samples were collected before gastrectomy, and CTCs were examined using a centrifugal microfluidic system with a new fluid-assisted separation technique. RESULTS: After creating a receiver operating characteristic curve to identify the discriminative CTC value needed differentiate patients with gastric cancer from healthy volunteers, sensitivity and specificity were nearly optimized at a CTC threshold of 2 per 7.5 mL of blood. Of the 102 persons with a CTC level ≥2 per 7.5 mL of blood, 99 (97.1%) had gastric cancer, and of the 45 persons with a CTC level <2 per 7.5 mL of blood, 28 (62.2%) were healthy controls. Accordingly, the sensitivity and specificity for the differentiation of patients with gastric cancer from healthy controls were 85.3% and 90.3%, respectively. However, the presence of CTCs was not associated with any clinicopathologic features such as staging, histologic type, or mucin phenotype. CONCLUSION: Although we could not prove the clinical feasibility of CTCs for gastric cancer staging, our results suggest a potential role of CTCs as an early diagnostic biomarker of gastric cancer. Public Library of Science 2017-06-29 /pmc/articles/PMC5491173/ /pubmed/28662130 http://dx.doi.org/10.1371/journal.pone.0180251 Text en © 2017 Kang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kang, Hwa Mi
Kim, Gwang Ha
Jeon, Hye Kyung
Kim, Dae Hwan
Jeon, Tae Yong
Park, Do Youn
Jeong, Hyunjin
Chun, Won Joo
Kim, Mi-Hyun
Park, Juhee
Lim, Minji
Kim, Tae-Hyeong
Cho, Yoon-Kyung
Circulating tumor cells detected by lab-on-a-disc: Role in early diagnosis of gastric cancer
title Circulating tumor cells detected by lab-on-a-disc: Role in early diagnosis of gastric cancer
title_full Circulating tumor cells detected by lab-on-a-disc: Role in early diagnosis of gastric cancer
title_fullStr Circulating tumor cells detected by lab-on-a-disc: Role in early diagnosis of gastric cancer
title_full_unstemmed Circulating tumor cells detected by lab-on-a-disc: Role in early diagnosis of gastric cancer
title_short Circulating tumor cells detected by lab-on-a-disc: Role in early diagnosis of gastric cancer
title_sort circulating tumor cells detected by lab-on-a-disc: role in early diagnosis of gastric cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491173/
https://www.ncbi.nlm.nih.gov/pubmed/28662130
http://dx.doi.org/10.1371/journal.pone.0180251
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