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Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model
European bat lyssavirus 1 is responsible for most bat rabies cases in Europe. Although EBLV-1 isolates display a high degree of sequence identity, different sublineages exist. In individual isolates various insertions and deletions have been identified, with unknown impact on viral replication and p...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491315/ https://www.ncbi.nlm.nih.gov/pubmed/28628617 http://dx.doi.org/10.1371/journal.pntd.0005668 |
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author | Eggerbauer, Elisa Pfaff, Florian Finke, Stefan Höper, Dirk Beer, Martin Mettenleiter, Thomas C. Nolden, Tobias Teifke, Jens-Peter Müller, Thomas Freuling, Conrad M. |
author_facet | Eggerbauer, Elisa Pfaff, Florian Finke, Stefan Höper, Dirk Beer, Martin Mettenleiter, Thomas C. Nolden, Tobias Teifke, Jens-Peter Müller, Thomas Freuling, Conrad M. |
author_sort | Eggerbauer, Elisa |
collection | PubMed |
description | European bat lyssavirus 1 is responsible for most bat rabies cases in Europe. Although EBLV-1 isolates display a high degree of sequence identity, different sublineages exist. In individual isolates various insertions and deletions have been identified, with unknown impact on viral replication and pathogenicity. In order to assess whether different genetic features of EBLV-1 isolates correlate with phenotypic changes, different EBLV-1 variants were compared for pathogenicity in the mouse model. Groups of three mice were infected intracranially (i.c.) with 10(2) TCID50/ml and groups of six mice were infected intramuscularly (i.m.) with 10(5) TCID50/ml and 10(2) TCID50/ml as well as intranasally (i.n.) with 10(2) TCID50/ml. Significant differences in survival following i.m. inoculation with low doses as well as i.n. inoculation were observed. Also, striking variations in incubation periods following i.c. inoculation and i.m. inoculation with high doses were seen. Hereby, the clinical picture differed between general symptoms, spasms and aggressiveness depending on the inoculation route. Immunohistochemistry of mouse brains showed that the virus distribution in the brain depended on the inoculation route. In conclusion, different EBLV-1 isolates differ in pathogenicity indicating variation which is not reflected in studies of single isolates. |
format | Online Article Text |
id | pubmed-5491315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54913152017-07-18 Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model Eggerbauer, Elisa Pfaff, Florian Finke, Stefan Höper, Dirk Beer, Martin Mettenleiter, Thomas C. Nolden, Tobias Teifke, Jens-Peter Müller, Thomas Freuling, Conrad M. PLoS Negl Trop Dis Research Article European bat lyssavirus 1 is responsible for most bat rabies cases in Europe. Although EBLV-1 isolates display a high degree of sequence identity, different sublineages exist. In individual isolates various insertions and deletions have been identified, with unknown impact on viral replication and pathogenicity. In order to assess whether different genetic features of EBLV-1 isolates correlate with phenotypic changes, different EBLV-1 variants were compared for pathogenicity in the mouse model. Groups of three mice were infected intracranially (i.c.) with 10(2) TCID50/ml and groups of six mice were infected intramuscularly (i.m.) with 10(5) TCID50/ml and 10(2) TCID50/ml as well as intranasally (i.n.) with 10(2) TCID50/ml. Significant differences in survival following i.m. inoculation with low doses as well as i.n. inoculation were observed. Also, striking variations in incubation periods following i.c. inoculation and i.m. inoculation with high doses were seen. Hereby, the clinical picture differed between general symptoms, spasms and aggressiveness depending on the inoculation route. Immunohistochemistry of mouse brains showed that the virus distribution in the brain depended on the inoculation route. In conclusion, different EBLV-1 isolates differ in pathogenicity indicating variation which is not reflected in studies of single isolates. Public Library of Science 2017-06-19 /pmc/articles/PMC5491315/ /pubmed/28628617 http://dx.doi.org/10.1371/journal.pntd.0005668 Text en © 2017 Eggerbauer et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Eggerbauer, Elisa Pfaff, Florian Finke, Stefan Höper, Dirk Beer, Martin Mettenleiter, Thomas C. Nolden, Tobias Teifke, Jens-Peter Müller, Thomas Freuling, Conrad M. Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model |
title | Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model |
title_full | Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model |
title_fullStr | Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model |
title_full_unstemmed | Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model |
title_short | Comparative analysis of European bat lyssavirus 1 pathogenicity in the mouse model |
title_sort | comparative analysis of european bat lyssavirus 1 pathogenicity in the mouse model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491315/ https://www.ncbi.nlm.nih.gov/pubmed/28628617 http://dx.doi.org/10.1371/journal.pntd.0005668 |
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