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The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants
The necrosis- and ethylene-inducing protein 1 (Nep1)-like proteins (NLPs) are a class of microbe-associated molecular patterns widely distributed across diverse groups of plant-associated microorganisms. In spite of the cytotoxic activity in dicot plants, the role of most NLPs in the virulence of pl...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491491/ https://www.ncbi.nlm.nih.gov/pubmed/28663588 http://dx.doi.org/10.1038/s41598-017-04430-0 |
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author | Fang, Ya-Li Peng, You-Liang Fan, Jun |
author_facet | Fang, Ya-Li Peng, You-Liang Fan, Jun |
author_sort | Fang, Ya-Li |
collection | PubMed |
description | The necrosis- and ethylene-inducing protein 1 (Nep1)-like proteins (NLPs) are a class of microbe-associated molecular patterns widely distributed across diverse groups of plant-associated microorganisms. In spite of the cytotoxic activity in dicot plants, the role of most NLPs in the virulence of plant pathogens is still largely unknown. We showed that the MoNLP family of rice blast fungus varied very little in amino acid sequence, transient expression of three MoNLPs induced cell death and the production of reactive oxygen species in Nicotiana benthamiana, and the expression of MoNLPs was induced during infection of susceptible rice plants. To further investigate the biological role of the MoNLP family, a marker-free gene replacement vector was developed and used to knock out the whole family in Magnaporthe oryzae. Results showed no significant difference in disease levels caused by wild type and the quadruple ΔMoNLP mutant strains. Likewise, the sporulation and radial growth of the two strains were similar under various unfavorable cultural conditions including malnutrition and abiotic stresses. These observations demonstrated that the MoNLP family is dispensable for the fungal tolerance to the tested adverse cultural conditions, and more importantly, for the virulence of blast fungus on susceptible rice plants. |
format | Online Article Text |
id | pubmed-5491491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54914912017-07-05 The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants Fang, Ya-Li Peng, You-Liang Fan, Jun Sci Rep Article The necrosis- and ethylene-inducing protein 1 (Nep1)-like proteins (NLPs) are a class of microbe-associated molecular patterns widely distributed across diverse groups of plant-associated microorganisms. In spite of the cytotoxic activity in dicot plants, the role of most NLPs in the virulence of plant pathogens is still largely unknown. We showed that the MoNLP family of rice blast fungus varied very little in amino acid sequence, transient expression of three MoNLPs induced cell death and the production of reactive oxygen species in Nicotiana benthamiana, and the expression of MoNLPs was induced during infection of susceptible rice plants. To further investigate the biological role of the MoNLP family, a marker-free gene replacement vector was developed and used to knock out the whole family in Magnaporthe oryzae. Results showed no significant difference in disease levels caused by wild type and the quadruple ΔMoNLP mutant strains. Likewise, the sporulation and radial growth of the two strains were similar under various unfavorable cultural conditions including malnutrition and abiotic stresses. These observations demonstrated that the MoNLP family is dispensable for the fungal tolerance to the tested adverse cultural conditions, and more importantly, for the virulence of blast fungus on susceptible rice plants. Nature Publishing Group UK 2017-06-29 /pmc/articles/PMC5491491/ /pubmed/28663588 http://dx.doi.org/10.1038/s41598-017-04430-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Fang, Ya-Li Peng, You-Liang Fan, Jun The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants |
title | The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants |
title_full | The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants |
title_fullStr | The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants |
title_full_unstemmed | The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants |
title_short | The Nep1-like protein family of Magnaporthe oryzae is dispensable for the infection of rice plants |
title_sort | nep1-like protein family of magnaporthe oryzae is dispensable for the infection of rice plants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491491/ https://www.ncbi.nlm.nih.gov/pubmed/28663588 http://dx.doi.org/10.1038/s41598-017-04430-0 |
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