Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase

In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulo...

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Autores principales: Donohoe, Bryon S., Wei, Hui, Mittal, Ashutosh, Shollenberger, Todd, Lunin, Vladimir V., Himmel, Michael E., Brunecky, Roman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491509/
https://www.ncbi.nlm.nih.gov/pubmed/28663545
http://dx.doi.org/10.1038/s41598-017-04502-1
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author Donohoe, Bryon S.
Wei, Hui
Mittal, Ashutosh
Shollenberger, Todd
Lunin, Vladimir V.
Himmel, Michael E.
Brunecky, Roman
author_facet Donohoe, Bryon S.
Wei, Hui
Mittal, Ashutosh
Shollenberger, Todd
Lunin, Vladimir V.
Himmel, Michael E.
Brunecky, Roman
author_sort Donohoe, Bryon S.
collection PubMed
description In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development.
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spelling pubmed-54915092017-07-05 Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase Donohoe, Bryon S. Wei, Hui Mittal, Ashutosh Shollenberger, Todd Lunin, Vladimir V. Himmel, Michael E. Brunecky, Roman Sci Rep Article In planta expression of a thermophilic endoglucanase (AcCel5A) reduces recalcitrance by creating voids and other irregularities in cell walls of Arabidopsis thaliana that increase enzyme accessibility without negative impacts on plant growth or cell wall composition. Our results suggest that cellulose β-1–4 linkages can be cut sparingly in the assembling wall and that these minimal changes, made at the proper time, have an impact on plant cell wall recalcitrance without negative effects on overall plant development. Nature Publishing Group UK 2017-06-29 /pmc/articles/PMC5491509/ /pubmed/28663545 http://dx.doi.org/10.1038/s41598-017-04502-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Donohoe, Bryon S.
Wei, Hui
Mittal, Ashutosh
Shollenberger, Todd
Lunin, Vladimir V.
Himmel, Michael E.
Brunecky, Roman
Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_full Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_fullStr Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_full_unstemmed Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_short Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
title_sort towards an understanding of enhanced biomass digestibility by in planta expression of a family 5 glycoside hydrolase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491509/
https://www.ncbi.nlm.nih.gov/pubmed/28663545
http://dx.doi.org/10.1038/s41598-017-04502-1
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