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Development and Use of a Real-Time Quantitative PCR Method for Detecting and Quantifying Equol-Producing Bacteria in Human Faecal Samples and Slurry Cultures
This work introduces a novel real-time quantitative PCR (qPCR) protocol for detecting and quantifying equol-producing bacteria. To this end, two sets of primers targeting the dihydrodaidzein reductase (ddr) and tetrahydrodaidzein reductase (tdr) genes, which are involved in the synthesis of equol, w...
Autores principales: | Vázquez, Lucía, Guadamuro, Lucía, Giganto, Froilán, Mayo, Baltasar, Flórez, Ana B. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491606/ https://www.ncbi.nlm.nih.gov/pubmed/28713336 http://dx.doi.org/10.3389/fmicb.2017.01155 |
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