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A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium

BACKGROUND: Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-...

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Autores principales: Moës, Elien, Vijgen, Leen, Keyaerts, Els, Zlateva, Kalina, Li, Sandra, Maes, Piet, Pyrc, Krzysztof, Berkhout, Ben, van der Hoek, Lia, Van Ranst, Marc
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549190/
https://www.ncbi.nlm.nih.gov/pubmed/15686594
http://dx.doi.org/10.1186/1471-2334-5-6
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author Moës, Elien
Vijgen, Leen
Keyaerts, Els
Zlateva, Kalina
Li, Sandra
Maes, Piet
Pyrc, Krzysztof
Berkhout, Ben
van der Hoek, Lia
Van Ranst, Marc
author_facet Moës, Elien
Vijgen, Leen
Keyaerts, Els
Zlateva, Kalina
Li, Sandra
Maes, Piet
Pyrc, Krzysztof
Berkhout, Ben
van der Hoek, Lia
Van Ranst, Marc
author_sort Moës, Elien
collection PubMed
description BACKGROUND: Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium. METHODS: Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay. RESULTS: Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004. CONCLUSIONS: Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating.
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spelling pubmed-5491902005-02-20 A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium Moës, Elien Vijgen, Leen Keyaerts, Els Zlateva, Kalina Li, Sandra Maes, Piet Pyrc, Krzysztof Berkhout, Ben van der Hoek, Lia Van Ranst, Marc BMC Infect Dis Research Article BACKGROUND: Four human coronaviruses are currently known to infect the respiratory tract: human coronaviruses OC43 (HCoV-OC43) and 229E (HCoV-229E), SARS associated coronavirus (SARS-CoV) and the recently identified human coronavirus NL63 (HCoV-NL63). In this study we explored the incidence of HCoV-NL63 infection in children diagnosed with respiratory tract infections in Belgium. METHODS: Samples from children hospitalized with respiratory diseases during the winter seasons of 2003 and 2004 were evaluated for the presence of HCoV-NL63 using a optimized pancoronavirus RT-PCR assay. RESULTS: Seven HCoV-NL63 positive samples were identified, six were collected during January/February 2003 and one at the end of February 2004. CONCLUSIONS: Our results support the notation that HCoV-NL63 can cause serious respiratory symptoms in children. Sequence analysis of the S gene showed that our isolates could be classified into two subtypes corresponding to the two prototype HCoV-NL63 sequences isolated in The Netherlands in 1988 and 2003, indicating that these two subtypes may currently be cocirculating. BioMed Central 2005-02-01 /pmc/articles/PMC549190/ /pubmed/15686594 http://dx.doi.org/10.1186/1471-2334-5-6 Text en Copyright © 2005 Moës et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Moës, Elien
Vijgen, Leen
Keyaerts, Els
Zlateva, Kalina
Li, Sandra
Maes, Piet
Pyrc, Krzysztof
Berkhout, Ben
van der Hoek, Lia
Van Ranst, Marc
A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
title A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
title_full A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
title_fullStr A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
title_full_unstemmed A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
title_short A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium
title_sort novel pancoronavirus rt-pcr assay: frequent detection of human coronavirus nl63 in children hospitalized with respiratory tract infections in belgium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549190/
https://www.ncbi.nlm.nih.gov/pubmed/15686594
http://dx.doi.org/10.1186/1471-2334-5-6
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