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Identification of Novel and Efficacious Chemical Compounds that Disturb Influenza A Virus Entry in vitro
Influenza A virus is a negative RNA stranded virus of the family Orthomyxoviridae, and represents a major public health threat, compounding existing disease conditions. Influenza A virus replicates rapidly within its host and the segmented nature of its genome facilitates re-assortment, whereby whol...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491913/ https://www.ncbi.nlm.nih.gov/pubmed/28713784 http://dx.doi.org/10.3389/fcimb.2017.00304 |
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author | Khalil, Hany El Malah, Tamer El Maksoud, Ahmed I. Abd El Halfawy, Ibrahim El Rashedy, Ahmed A. El Hefnawy, Mahmoud |
author_facet | Khalil, Hany El Malah, Tamer El Maksoud, Ahmed I. Abd El Halfawy, Ibrahim El Rashedy, Ahmed A. El Hefnawy, Mahmoud |
author_sort | Khalil, Hany |
collection | PubMed |
description | Influenza A virus is a negative RNA stranded virus of the family Orthomyxoviridae, and represents a major public health threat, compounding existing disease conditions. Influenza A virus replicates rapidly within its host and the segmented nature of its genome facilitates re-assortment, whereby whole genes are exchanged between influenza virus subtypes during replication. Antiviral medications are important pharmacological tools in influenza virus prophylaxis and therapy. However, the use of currently available antiviral is impeded by sometimes high levels of resistance in circulating virus strains. Here, we identified novel anti-influenza compounds through screening of chemical compounds synthesized de novo on human lung epithelial cells. Computational and experimental screening of extensive and water soluble compounds identified novel influenza virus inhibitors that can reduce influenza virus infection without detectable toxic effects on host cells. Interestingly, the indicated active compounds inhibit viral replication most likely via interaction with cell receptors and disturb influenza virus entry into host cells. Collectively, screening of new synthesis chemical compounds on influenza A virus replication provides a novel and efficacious anti-influenza compounds that can inhibit viral replication via disturbing virus entry and indicates that these compounds are attractive candidates for evaluation as potential anti-influenza drugs. |
format | Online Article Text |
id | pubmed-5491913 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54919132017-07-14 Identification of Novel and Efficacious Chemical Compounds that Disturb Influenza A Virus Entry in vitro Khalil, Hany El Malah, Tamer El Maksoud, Ahmed I. Abd El Halfawy, Ibrahim El Rashedy, Ahmed A. El Hefnawy, Mahmoud Front Cell Infect Microbiol Microbiology Influenza A virus is a negative RNA stranded virus of the family Orthomyxoviridae, and represents a major public health threat, compounding existing disease conditions. Influenza A virus replicates rapidly within its host and the segmented nature of its genome facilitates re-assortment, whereby whole genes are exchanged between influenza virus subtypes during replication. Antiviral medications are important pharmacological tools in influenza virus prophylaxis and therapy. However, the use of currently available antiviral is impeded by sometimes high levels of resistance in circulating virus strains. Here, we identified novel anti-influenza compounds through screening of chemical compounds synthesized de novo on human lung epithelial cells. Computational and experimental screening of extensive and water soluble compounds identified novel influenza virus inhibitors that can reduce influenza virus infection without detectable toxic effects on host cells. Interestingly, the indicated active compounds inhibit viral replication most likely via interaction with cell receptors and disturb influenza virus entry into host cells. Collectively, screening of new synthesis chemical compounds on influenza A virus replication provides a novel and efficacious anti-influenza compounds that can inhibit viral replication via disturbing virus entry and indicates that these compounds are attractive candidates for evaluation as potential anti-influenza drugs. Frontiers Media S.A. 2017-06-30 /pmc/articles/PMC5491913/ /pubmed/28713784 http://dx.doi.org/10.3389/fcimb.2017.00304 Text en Copyright © 2017 Khalil, El Malah, El Maksoud, El Halfawy, El Rashedy and El Hefnawy. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Khalil, Hany El Malah, Tamer El Maksoud, Ahmed I. Abd El Halfawy, Ibrahim El Rashedy, Ahmed A. El Hefnawy, Mahmoud Identification of Novel and Efficacious Chemical Compounds that Disturb Influenza A Virus Entry in vitro |
title | Identification of Novel and Efficacious Chemical Compounds that Disturb Influenza A Virus Entry in vitro |
title_full | Identification of Novel and Efficacious Chemical Compounds that Disturb Influenza A Virus Entry in vitro |
title_fullStr | Identification of Novel and Efficacious Chemical Compounds that Disturb Influenza A Virus Entry in vitro |
title_full_unstemmed | Identification of Novel and Efficacious Chemical Compounds that Disturb Influenza A Virus Entry in vitro |
title_short | Identification of Novel and Efficacious Chemical Compounds that Disturb Influenza A Virus Entry in vitro |
title_sort | identification of novel and efficacious chemical compounds that disturb influenza a virus entry in vitro |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5491913/ https://www.ncbi.nlm.nih.gov/pubmed/28713784 http://dx.doi.org/10.3389/fcimb.2017.00304 |
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