In vitro bioassay as a predictor of in vivo response

BACKGROUND: There is a substantial discrepancy between in vitro and in vivo experiments. The purpose of the present work was development of a theoretical framework to enable improved prediction of in vivo response from in vitro bioassay results. RESULTS: For dose-response curve reaches a plateau in...

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Detalles Bibliográficos
Autores principales: Barnard, Ross, Gurevich, Konstantin G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549212/
https://www.ncbi.nlm.nih.gov/pubmed/15698478
http://dx.doi.org/10.1186/1742-4682-2-3
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author Barnard, Ross
Gurevich, Konstantin G
author_facet Barnard, Ross
Gurevich, Konstantin G
author_sort Barnard, Ross
collection PubMed
description BACKGROUND: There is a substantial discrepancy between in vitro and in vivo experiments. The purpose of the present work was development of a theoretical framework to enable improved prediction of in vivo response from in vitro bioassay results. RESULTS: For dose-response curve reaches a plateau in vitro we demonstrated that the in vivo response has only one maximum. For biphasic patterns of biological response in vitro both the bimodal and biphasic in vivo responses might be observed. CONCLUSION: As the main result of this work we have demonstrated that in vivo responses might be predicted from dose-effect curves measured in vitro.
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spelling pubmed-5492122005-02-23 In vitro bioassay as a predictor of in vivo response Barnard, Ross Gurevich, Konstantin G Theor Biol Med Model Research BACKGROUND: There is a substantial discrepancy between in vitro and in vivo experiments. The purpose of the present work was development of a theoretical framework to enable improved prediction of in vivo response from in vitro bioassay results. RESULTS: For dose-response curve reaches a plateau in vitro we demonstrated that the in vivo response has only one maximum. For biphasic patterns of biological response in vitro both the bimodal and biphasic in vivo responses might be observed. CONCLUSION: As the main result of this work we have demonstrated that in vivo responses might be predicted from dose-effect curves measured in vitro. BioMed Central 2005-02-07 /pmc/articles/PMC549212/ /pubmed/15698478 http://dx.doi.org/10.1186/1742-4682-2-3 Text en Copyright © 2005 Barnard and Gurevich; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Barnard, Ross
Gurevich, Konstantin G
In vitro bioassay as a predictor of in vivo response
title In vitro bioassay as a predictor of in vivo response
title_full In vitro bioassay as a predictor of in vivo response
title_fullStr In vitro bioassay as a predictor of in vivo response
title_full_unstemmed In vitro bioassay as a predictor of in vivo response
title_short In vitro bioassay as a predictor of in vivo response
title_sort in vitro bioassay as a predictor of in vivo response
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549212/
https://www.ncbi.nlm.nih.gov/pubmed/15698478
http://dx.doi.org/10.1186/1742-4682-2-3
work_keys_str_mv AT barnardross invitrobioassayasapredictorofinvivoresponse
AT gurevichkonstanting invitrobioassayasapredictorofinvivoresponse

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