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HIV-1 Tat interacts with LIS1 protein

BACKGROUND: HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-...

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Autores principales: Epie, Nicolas, Ammosova, Tatyana, Sapir, Tamar, Voloshin, Yaroslav, Lane, William S, Turner, Willie, Reiner, Orly, Nekhai, Sergei
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549217/
https://www.ncbi.nlm.nih.gov/pubmed/15698475
http://dx.doi.org/10.1186/1742-4690-2-6
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author Epie, Nicolas
Ammosova, Tatyana
Sapir, Tamar
Voloshin, Yaroslav
Lane, William S
Turner, Willie
Reiner, Orly
Nekhai, Sergei
author_facet Epie, Nicolas
Ammosova, Tatyana
Sapir, Tamar
Voloshin, Yaroslav
Lane, William S
Turner, Willie
Reiner, Orly
Nekhai, Sergei
author_sort Epie, Nicolas
collection PubMed
description BACKGROUND: HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization. RESULTS: We identified here LIS1 as a Tat-interacting protein during extensive biochemical fractionation of T-cell extracts. We found several proteins to co-purify with a Tat-associated RNAPII CTD kinase activity including LIS1, CDK7, cyclin H, and MAT1. Tat interacted with LIS1 but not with CDK7, cyclin H or MAT1 in vitro. LIS1 also co-immunoprecipitated with Tat expressed in HeLa cells. Further, LIS1 interacted with Tat in a yeast two-hybrid system. CONCLUSION: Our results indicate that Tat interacts with LIS1 in vitro and in vivo and that this interaction might contribute to the effect of Tat on microtubule formation.
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spelling pubmed-5492172005-02-23 HIV-1 Tat interacts with LIS1 protein Epie, Nicolas Ammosova, Tatyana Sapir, Tamar Voloshin, Yaroslav Lane, William S Turner, Willie Reiner, Orly Nekhai, Sergei Retrovirology Research BACKGROUND: HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization. RESULTS: We identified here LIS1 as a Tat-interacting protein during extensive biochemical fractionation of T-cell extracts. We found several proteins to co-purify with a Tat-associated RNAPII CTD kinase activity including LIS1, CDK7, cyclin H, and MAT1. Tat interacted with LIS1 but not with CDK7, cyclin H or MAT1 in vitro. LIS1 also co-immunoprecipitated with Tat expressed in HeLa cells. Further, LIS1 interacted with Tat in a yeast two-hybrid system. CONCLUSION: Our results indicate that Tat interacts with LIS1 in vitro and in vivo and that this interaction might contribute to the effect of Tat on microtubule formation. BioMed Central 2005-02-07 /pmc/articles/PMC549217/ /pubmed/15698475 http://dx.doi.org/10.1186/1742-4690-2-6 Text en Copyright © 2005 Epie et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Epie, Nicolas
Ammosova, Tatyana
Sapir, Tamar
Voloshin, Yaroslav
Lane, William S
Turner, Willie
Reiner, Orly
Nekhai, Sergei
HIV-1 Tat interacts with LIS1 protein
title HIV-1 Tat interacts with LIS1 protein
title_full HIV-1 Tat interacts with LIS1 protein
title_fullStr HIV-1 Tat interacts with LIS1 protein
title_full_unstemmed HIV-1 Tat interacts with LIS1 protein
title_short HIV-1 Tat interacts with LIS1 protein
title_sort hiv-1 tat interacts with lis1 protein
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC549217/
https://www.ncbi.nlm.nih.gov/pubmed/15698475
http://dx.doi.org/10.1186/1742-4690-2-6
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