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Design and Performance of a Portable and Multichannel SPR Device
A portable multichannel surface plasmon resonance (SPR) biosensor device is presented in this study. As an optical biosensor device, the core component of its light path is a semi-cylindrical prism, which is used as the coupling unit for the excitation of the SPR phenomena. Based on this prism, a we...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5492262/ https://www.ncbi.nlm.nih.gov/pubmed/28629186 http://dx.doi.org/10.3390/s17061435 |
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author | Zhang, Xiao-ling Liu, Yan Fan, Ting Hu, Ning Yang, Zhong Chen, Xi Wang, Zhen-yu Yang, Jun |
author_facet | Zhang, Xiao-ling Liu, Yan Fan, Ting Hu, Ning Yang, Zhong Chen, Xi Wang, Zhen-yu Yang, Jun |
author_sort | Zhang, Xiao-ling |
collection | PubMed |
description | A portable multichannel surface plasmon resonance (SPR) biosensor device is presented in this study. As an optical biosensor device, the core component of its light path is a semi-cylindrical prism, which is used as the coupling unit for the excitation of the SPR phenomena. Based on this prism, a wedge-shaped incident light beam including a continuous angle range (10°) is chosen to replace the commonly-used parallel light beam in traditional SPR devices, in which the incident angle is adjusted by a sophisticated mechanical system. Thus, complicated, cumbersome, and costly mechanical structures can be avoided in this design. Furthermore, the selection of a small and high-stability semiconductor laser and matrix CCD detector as well as a microfluidic system aids in the realization of a miniaturized and multichannel device. Several different samples were used to test the performance of this new device. For ethanol with different concentrations, the sensing response was of good linear relativity with the concentration (Y = 3.17143X + 2.81518, R(2) = 0.97661). Mouse IgG and goat anti-mouse IgG were used as biological samples for immunological analysis, and BSA as the control group. Good specific recognition between mouse IgG and goat anti-mouse IgG has been achieved. The detection limit of antibody to antigen coated on the sensing surface was about 25 mg/L without surface modification. |
format | Online Article Text |
id | pubmed-5492262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54922622017-07-03 Design and Performance of a Portable and Multichannel SPR Device Zhang, Xiao-ling Liu, Yan Fan, Ting Hu, Ning Yang, Zhong Chen, Xi Wang, Zhen-yu Yang, Jun Sensors (Basel) Article A portable multichannel surface plasmon resonance (SPR) biosensor device is presented in this study. As an optical biosensor device, the core component of its light path is a semi-cylindrical prism, which is used as the coupling unit for the excitation of the SPR phenomena. Based on this prism, a wedge-shaped incident light beam including a continuous angle range (10°) is chosen to replace the commonly-used parallel light beam in traditional SPR devices, in which the incident angle is adjusted by a sophisticated mechanical system. Thus, complicated, cumbersome, and costly mechanical structures can be avoided in this design. Furthermore, the selection of a small and high-stability semiconductor laser and matrix CCD detector as well as a microfluidic system aids in the realization of a miniaturized and multichannel device. Several different samples were used to test the performance of this new device. For ethanol with different concentrations, the sensing response was of good linear relativity with the concentration (Y = 3.17143X + 2.81518, R(2) = 0.97661). Mouse IgG and goat anti-mouse IgG were used as biological samples for immunological analysis, and BSA as the control group. Good specific recognition between mouse IgG and goat anti-mouse IgG has been achieved. The detection limit of antibody to antigen coated on the sensing surface was about 25 mg/L without surface modification. MDPI 2017-06-19 /pmc/articles/PMC5492262/ /pubmed/28629186 http://dx.doi.org/10.3390/s17061435 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Xiao-ling Liu, Yan Fan, Ting Hu, Ning Yang, Zhong Chen, Xi Wang, Zhen-yu Yang, Jun Design and Performance of a Portable and Multichannel SPR Device |
title | Design and Performance of a Portable and Multichannel SPR Device |
title_full | Design and Performance of a Portable and Multichannel SPR Device |
title_fullStr | Design and Performance of a Portable and Multichannel SPR Device |
title_full_unstemmed | Design and Performance of a Portable and Multichannel SPR Device |
title_short | Design and Performance of a Portable and Multichannel SPR Device |
title_sort | design and performance of a portable and multichannel spr device |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5492262/ https://www.ncbi.nlm.nih.gov/pubmed/28629186 http://dx.doi.org/10.3390/s17061435 |
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