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Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic

Faecalibacterium prausnitzii is a major member of the Firmicutes phylum and one of the most abundant bacteria in the healthy human microbiota. F. prausnitzii depletion has been reported in several intestinal disorders, and more consistently in Crohn's disease (CD) patients. Despite its importan...

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Autores principales: Martín, Rebeca, Miquel, Sylvie, Benevides, Leandro, Bridonneau, Chantal, Robert, Véronique, Hudault, Sylvie, Chain, Florian, Berteau, Olivier, Azevedo, Vasco, Chatel, Jean M., Sokol, Harry, Bermúdez-Humarán, Luis G., Thomas, Muriel, Langella, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5492426/
https://www.ncbi.nlm.nih.gov/pubmed/28713353
http://dx.doi.org/10.3389/fmicb.2017.01226
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author Martín, Rebeca
Miquel, Sylvie
Benevides, Leandro
Bridonneau, Chantal
Robert, Véronique
Hudault, Sylvie
Chain, Florian
Berteau, Olivier
Azevedo, Vasco
Chatel, Jean M.
Sokol, Harry
Bermúdez-Humarán, Luis G.
Thomas, Muriel
Langella, Philippe
author_facet Martín, Rebeca
Miquel, Sylvie
Benevides, Leandro
Bridonneau, Chantal
Robert, Véronique
Hudault, Sylvie
Chain, Florian
Berteau, Olivier
Azevedo, Vasco
Chatel, Jean M.
Sokol, Harry
Bermúdez-Humarán, Luis G.
Thomas, Muriel
Langella, Philippe
author_sort Martín, Rebeca
collection PubMed
description Faecalibacterium prausnitzii is a major member of the Firmicutes phylum and one of the most abundant bacteria in the healthy human microbiota. F. prausnitzii depletion has been reported in several intestinal disorders, and more consistently in Crohn's disease (CD) patients. Despite its importance in human health, only few microbiological studies have been performed to isolate novel F. prausnitzii strains in order to better understand the biodiversity and physiological diversity of this beneficial commensal species. In this study, we described a protocol to isolate novel F. prausnitzii strains from feces of healthy volunteers as well as a deep molecular and metabolic characterization of these isolated strains. These F. prausnitzii strains were classified in two phylogroups and three clusters according to 16S rRNA sequences and results support that they would belong to two different genomospecies or genomovars as no genome sequencing has been performed in this work. Differences in enzymes production, antibiotic resistance and immunomodulatory properties were found to be strain-dependent. So far, all F. prausnitzii isolates share some characteristic such as (i) the lack of epithelial cells adhesion, plasmids, anti-microbial, and hemolytic activity and (ii) the presence of DNAse activity. Furthermore, Short Chain Fatty Acids (SCFA) production was assessed for the novel isolates as these products influence intestinal homeostasis. Indeed, the butyrate production has been correlated to the capacity to induce IL-10, an anti-inflammatory cytokine, in peripheral blood mononuclear cells (PBMC) but not to the ability to block IL-8 secretion in TNF-α-stimulated HT-29 cells, reinforcing the hypothesis of a complex anti-inflammatory pathway driven by F. prausnitzii. Altogether, our results suggest that some F. prausnitzii strains could represent good candidates as next-generation probiotic.
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spelling pubmed-54924262017-07-14 Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic Martín, Rebeca Miquel, Sylvie Benevides, Leandro Bridonneau, Chantal Robert, Véronique Hudault, Sylvie Chain, Florian Berteau, Olivier Azevedo, Vasco Chatel, Jean M. Sokol, Harry Bermúdez-Humarán, Luis G. Thomas, Muriel Langella, Philippe Front Microbiol Microbiology Faecalibacterium prausnitzii is a major member of the Firmicutes phylum and one of the most abundant bacteria in the healthy human microbiota. F. prausnitzii depletion has been reported in several intestinal disorders, and more consistently in Crohn's disease (CD) patients. Despite its importance in human health, only few microbiological studies have been performed to isolate novel F. prausnitzii strains in order to better understand the biodiversity and physiological diversity of this beneficial commensal species. In this study, we described a protocol to isolate novel F. prausnitzii strains from feces of healthy volunteers as well as a deep molecular and metabolic characterization of these isolated strains. These F. prausnitzii strains were classified in two phylogroups and three clusters according to 16S rRNA sequences and results support that they would belong to two different genomospecies or genomovars as no genome sequencing has been performed in this work. Differences in enzymes production, antibiotic resistance and immunomodulatory properties were found to be strain-dependent. So far, all F. prausnitzii isolates share some characteristic such as (i) the lack of epithelial cells adhesion, plasmids, anti-microbial, and hemolytic activity and (ii) the presence of DNAse activity. Furthermore, Short Chain Fatty Acids (SCFA) production was assessed for the novel isolates as these products influence intestinal homeostasis. Indeed, the butyrate production has been correlated to the capacity to induce IL-10, an anti-inflammatory cytokine, in peripheral blood mononuclear cells (PBMC) but not to the ability to block IL-8 secretion in TNF-α-stimulated HT-29 cells, reinforcing the hypothesis of a complex anti-inflammatory pathway driven by F. prausnitzii. Altogether, our results suggest that some F. prausnitzii strains could represent good candidates as next-generation probiotic. Frontiers Media S.A. 2017-06-30 /pmc/articles/PMC5492426/ /pubmed/28713353 http://dx.doi.org/10.3389/fmicb.2017.01226 Text en Copyright © 2017 Martín, Miquel, Benevides, Bridonneau, Robert, Hudault, Chain, Berteau, Azevedo, Chatel, Sokol, Bermúdez-Humarán, Thomas and Langella. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Martín, Rebeca
Miquel, Sylvie
Benevides, Leandro
Bridonneau, Chantal
Robert, Véronique
Hudault, Sylvie
Chain, Florian
Berteau, Olivier
Azevedo, Vasco
Chatel, Jean M.
Sokol, Harry
Bermúdez-Humarán, Luis G.
Thomas, Muriel
Langella, Philippe
Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic
title Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic
title_full Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic
title_fullStr Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic
title_full_unstemmed Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic
title_short Functional Characterization of Novel Faecalibacterium prausnitzii Strains Isolated from Healthy Volunteers: A Step Forward in the Use of F. prausnitzii as a Next-Generation Probiotic
title_sort functional characterization of novel faecalibacterium prausnitzii strains isolated from healthy volunteers: a step forward in the use of f. prausnitzii as a next-generation probiotic
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5492426/
https://www.ncbi.nlm.nih.gov/pubmed/28713353
http://dx.doi.org/10.3389/fmicb.2017.01226
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