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Exposure to Umbelliferone Reduces Ralstonia solanacearum Biofilm Formation, Transcription of Type III Secretion System Regulators and Effectors and Virulence on Tobacco

Ralstonia solanacearum is one of the most devastating phytopathogens and causes bacterial wilt, which leads to severe economic loss due to its worldwide distribution and broad host range. Certain plant-derived compounds (PDCs) can impair bacterial virulence by suppressing pathogenic factors of R. so...

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Autores principales: Yang, Liang, Li, Shili, Qin, Xiyun, Jiang, Gaofei, Chen, Juanni, Li, Bide, Yao, Xiaoyuan, Liang, Peibo, Zhang, Yong, Ding, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5492427/
https://www.ncbi.nlm.nih.gov/pubmed/28713361
http://dx.doi.org/10.3389/fmicb.2017.01234
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author Yang, Liang
Li, Shili
Qin, Xiyun
Jiang, Gaofei
Chen, Juanni
Li, Bide
Yao, Xiaoyuan
Liang, Peibo
Zhang, Yong
Ding, Wei
author_facet Yang, Liang
Li, Shili
Qin, Xiyun
Jiang, Gaofei
Chen, Juanni
Li, Bide
Yao, Xiaoyuan
Liang, Peibo
Zhang, Yong
Ding, Wei
author_sort Yang, Liang
collection PubMed
description Ralstonia solanacearum is one of the most devastating phytopathogens and causes bacterial wilt, which leads to severe economic loss due to its worldwide distribution and broad host range. Certain plant-derived compounds (PDCs) can impair bacterial virulence by suppressing pathogenic factors of R. solanacearum. However, the inhibitory mechanisms of PDCs in bacterial virulence remain largely unknown. In this study, we screened a library of coumarins and derivatives, natural PDCs with fused benzene and α-pyrone rings, for their effects on expression of the type III secretion system (T3SS) of R. solanacearum. Here, we show that umbelliferone (UM), a 7-hydroxycoumarin, suppressed T3SS regulator gene expression through HrpG–HrpB and PrhG–HrpB pathways. UM decreased gene expression of six type III effectors (RipX, RipD, RipP1, RipR, RipTAL, and RipW) of 10 representative effector genes but did not alter T2SS expression. In addition, biofilm formation of R. solanacearum was significantly reduced by UM, though swimming activity was not affected. We then observed that UM suppressed the wilting disease process by reducing colonization and proliferation in tobacco roots and stems. In summary, the findings reveal that UM may serve as a plant-derived inhibitor to manipulate R. solanacearum T3SS and biofilm formation, providing proof of concept that these key virulence factors are potential targets for the integrated control of bacterial wilt.
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spelling pubmed-54924272017-07-14 Exposure to Umbelliferone Reduces Ralstonia solanacearum Biofilm Formation, Transcription of Type III Secretion System Regulators and Effectors and Virulence on Tobacco Yang, Liang Li, Shili Qin, Xiyun Jiang, Gaofei Chen, Juanni Li, Bide Yao, Xiaoyuan Liang, Peibo Zhang, Yong Ding, Wei Front Microbiol Microbiology Ralstonia solanacearum is one of the most devastating phytopathogens and causes bacterial wilt, which leads to severe economic loss due to its worldwide distribution and broad host range. Certain plant-derived compounds (PDCs) can impair bacterial virulence by suppressing pathogenic factors of R. solanacearum. However, the inhibitory mechanisms of PDCs in bacterial virulence remain largely unknown. In this study, we screened a library of coumarins and derivatives, natural PDCs with fused benzene and α-pyrone rings, for their effects on expression of the type III secretion system (T3SS) of R. solanacearum. Here, we show that umbelliferone (UM), a 7-hydroxycoumarin, suppressed T3SS regulator gene expression through HrpG–HrpB and PrhG–HrpB pathways. UM decreased gene expression of six type III effectors (RipX, RipD, RipP1, RipR, RipTAL, and RipW) of 10 representative effector genes but did not alter T2SS expression. In addition, biofilm formation of R. solanacearum was significantly reduced by UM, though swimming activity was not affected. We then observed that UM suppressed the wilting disease process by reducing colonization and proliferation in tobacco roots and stems. In summary, the findings reveal that UM may serve as a plant-derived inhibitor to manipulate R. solanacearum T3SS and biofilm formation, providing proof of concept that these key virulence factors are potential targets for the integrated control of bacterial wilt. Frontiers Media S.A. 2017-06-30 /pmc/articles/PMC5492427/ /pubmed/28713361 http://dx.doi.org/10.3389/fmicb.2017.01234 Text en Copyright © 2017 Yang, Li, Qin, Jiang, Chen, Li, Yao, Liang, Zhang and Ding. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yang, Liang
Li, Shili
Qin, Xiyun
Jiang, Gaofei
Chen, Juanni
Li, Bide
Yao, Xiaoyuan
Liang, Peibo
Zhang, Yong
Ding, Wei
Exposure to Umbelliferone Reduces Ralstonia solanacearum Biofilm Formation, Transcription of Type III Secretion System Regulators and Effectors and Virulence on Tobacco
title Exposure to Umbelliferone Reduces Ralstonia solanacearum Biofilm Formation, Transcription of Type III Secretion System Regulators and Effectors and Virulence on Tobacco
title_full Exposure to Umbelliferone Reduces Ralstonia solanacearum Biofilm Formation, Transcription of Type III Secretion System Regulators and Effectors and Virulence on Tobacco
title_fullStr Exposure to Umbelliferone Reduces Ralstonia solanacearum Biofilm Formation, Transcription of Type III Secretion System Regulators and Effectors and Virulence on Tobacco
title_full_unstemmed Exposure to Umbelliferone Reduces Ralstonia solanacearum Biofilm Formation, Transcription of Type III Secretion System Regulators and Effectors and Virulence on Tobacco
title_short Exposure to Umbelliferone Reduces Ralstonia solanacearum Biofilm Formation, Transcription of Type III Secretion System Regulators and Effectors and Virulence on Tobacco
title_sort exposure to umbelliferone reduces ralstonia solanacearum biofilm formation, transcription of type iii secretion system regulators and effectors and virulence on tobacco
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5492427/
https://www.ncbi.nlm.nih.gov/pubmed/28713361
http://dx.doi.org/10.3389/fmicb.2017.01234
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