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An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes
This study investigates the challenge of comprehensively cataloging the complete human proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics. We modify a classical two-dimensional high-resolution reversed-phase peptide fractionation scheme and optimize a protocol tha...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5493283/ https://www.ncbi.nlm.nih.gov/pubmed/28601559 http://dx.doi.org/10.1016/j.cels.2017.05.009 |